| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAMD4A |
| Uniprot No | Q9UPU9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-718 aa |
| 活性数据 | MMFRDQVGVL AGWFKGWNEC EQTVALLSLL KRVSQTQARF LQLCLEHSLA DCAELHVLER EANSPGIINQ WQQESKDKVI SLLLTHLPLL KPGNLDAKVE YMKLLPKILA HSIEHNQHIE ESRQLLSYAL IHPATSLEDR SALAMWLNHL EDRTSTSFGG QNRGRSDSVD YGQTHYYHQR QNSDDKLNGW QNSRDSGICI NASNWQDKSM GCENGHVPLY SSSSVPTTIN TIGTSTSTIL SGQAHHSPLK RSVSLTPPMN VPNQPLGHGW MSHEDLRARG PQCLPSDHAP LSPQSSVASS GSGGSEHLED QTTARNTFQE EGSGMKDVPA WLKSLRLHKY AALFSQMTYE EMMALTECQL EAQNVTKGAR HKIVISIQKL KERQNLLKSL ERDIIEGGSL RIPLQELHQM ILTPIKAYSS PSTTPEARRR EPQAPRQPSL MGPESQSPDC KDGAAATGAT ATPSAGASGG LQPHQLSSCD GELAVAPLPE GDLPGQFTRV MGKVCTQLLV SRPDEENISS YLQLIDKCLI HEAFTETQKK RLLSWKQQVQ KLFRSFPRKT LLDISGYRQQ RNRGFGQSNS LPTAGSVGGG MGRRNPRQYQ IPSRNVPSAR LGLLGTSGFV SSNQRNTTAT PTIMKQGRQN LWFANPGGSN SMPSRTHSSV QRTRSLPVHT SPQNMLMFQQ PEFQLPVTEP DINNRLESLC LSMTEHALGD GVDRTSTI |
| 分子量 | 79.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SAMD4A蛋白的3篇代表性文献(基于近年研究热点构造的示例性内容，实际文献需通过数据库验证)：
1. **文献名称**: *Structural and functional analysis of SAMD4A in RNA binding regulation*
**作者**: Zhang, Y. et al. (2018)
**摘要**: 通过重组表达人源SAMD4A蛋白，解析其SAM结构域与RNA结合的分子机制，发现其对神经元mRNA翻译的抑制作用，为神经退行性疾病研究提供新靶点。
2. **文献名称**: *SAMD4A modulates cellular stress responses by regulating mRNA translation*
**作者**: Lee, J. & Smith, C. (2020)
**摘要**: 利用重组SAMD4A蛋白验证其在细胞应激颗粒组装中的关键作用，证明其通过结合特定RNA序列抑制蛋白合成，参与ALS(肌萎缩侧索硬化)病理模型。
3. **文献名称**: *SAMD4A as a novel antiviral host factor: Insights from recombinant protein assays*
**作者**: Kumar, R. & Li, X. (2021)
**摘要**: 研究重组SAMD4A蛋白与病毒RNA的互作，揭示其通过限制病毒mRNA翻译抑制流感病毒复制的分子通路，提出其作为广谱抗病毒治疗潜力。
*注：以上内容综合了SAMD4A已知功能(RNA结合、翻译调控、疾病关联)，具体文献需根据实际研究补充。建议通过PubMed或Web of Science搜索关键词"SAMD4A recombinant"进一步筛选。*
**Background of Recombinant Human SAMD4A Protein**
SAMD4A (Sterile Alpha Motif Domain-containing 4A) is a conserved RNA-binding protein belonging to the SAMD family, characterized by a Sterile Alpha Motif (SAM) domain crucial for protein-protein interactions and a Smad-binding domain. It plays regulatory roles in post-transcriptional gene expression, primarily by binding to specific mRNA motifs (e.g., in the 3' UTR) to control translation efficiency or stability. SAMD4A is implicated in cellular processes like stress response, differentiation, and apoptosis. Studies highlight its involvement in mTOR signaling and stress granule dynamics, linking it to metabolic and proteostatic regulation.
Dysregulation of SAMD4A is associated with diseases, including cancers (e.g., glioblastoma, breast cancer) and neurological disorders. For instance, it acts as a tumor suppressor by inhibiting pro-survival mRNA translation. Recombinant human SAMD4A (rhSAMD4A), produced via bacterial or mammalian expression systems, retains these functional domains, enabling in vitro studies of its RNA-binding properties, interaction partners, and regulatory mechanisms. Its applications span investigating molecular pathways in disease models, screening therapeutic compounds, and developing RNA-targeted therapies. Ongoing research explores SAMD4A’s potential as a biomarker or therapeutic target, emphasizing its dual roles in cellular homeostasis and pathology.
×
