| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEC24C |
| Uniprot No | P53992 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1094 aa |
| 活性数据 | MNVNQSVPPVPPFGQPQPIYPGYHQSSYGGQSGSTAPAIPYGAYNGPVPGYQQTPPQGMSRAPPSSGAPPASTAQAPCGQAAYGQFGQGDVQNGPSSTVQMQRLPGSQSFGSPLAPVGNQPPVLQPYGPPPTSAQVATQLSGMQISGAVAPAPPSSGLGFGPPTSLASASGSFPNSGLYGSYPQGQAPPLSQAQGHPGIQTPQRSAPSQASSFTPPASGGPRLPSMTGPLLPGQSFGGPSVSQPNHVSSPPQALPPGTQMTGPLGPLPPMHSPQQPGYQPQQNGSFGPARGPQSNYGGPYPAAPTFGSQPGPPQPLPPKRLDPDAIPSPIQVIEDDRNNRGTEPFVTGVRGQVPPLVTTNFLVKDQGNASPRYIRCTSYNIPCTSDMAKQAQVPLAAVIKPLARLPPEEASPYVVDHGESGPLRCNRCKAYMCPFMQFIEGGRRFQCCFCSCINDVPPQYFQHLDHTGKRVDAYDRPELSLGSYEFLATVDYCKNNKFPSPPAFIFMIDVSYNAIRTGLVRLLCEELKSLLDFLPREGGAEESAIRVGFVTYNKVLHFYNVKSSLAQPQMMVVSDVADMFVPLLDGFLVNVNESRAVITSLLDQIPEMFADTRETETVFVPVIQAGMEALKAAECAGKLFLFHTSLPIAEAPGKLKNRDDRKLINTDKEKTLFQPQTGAYQTLAKECVAQGCCVDLFLFPNQYVDVATLSVVPQLTGGSVYKYASFQVENDQERFLSDLRRDVQKVVGFDAVMRVRTSTGIRAVDFFGAFYMSNTTDVELAGLDGDKTVTVEFKHDDRLNEESGALLQCALLYTSCAGQRRLRIHNLALNCCTQLADLYRNCETDTLINYMAKFAYRGVLNSPVKAVRDTLITQCAQILACYRKNCASPSSAGQLILPECMKLLPVYLNCVLKSDVLQPGAEVTTDDRAYVRQLVTSMDVTETNVFFYPRLLPLTKSPVESTTEPPAVRASEERLSNGDIYLLENGLNLFLWVGASVQQGVVQSLFSVSSFSQITSGLSVLPVLDNPLSKKVRGLIDSLRAQRSRYMKLTVVKQEDKMEMLFKHFLVEDKSLSGGASYVDFLCHMHKEIRQLLS |
| 分子量 | 145.86 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人SEC24C蛋白相关的3篇文献摘要(信息基于虚构整合，供参考)：
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1. **文献名称**：*Structural insights into human SEC24C-mediated cargo selection in COPII vesicles*
**作者**：Zhang, Y., et al.
**摘要**：该研究解析了人源SEC24C蛋白的晶体结构，揭示其与COPII复合体中其他亚基的相互作用机制，发现SEC24C通过特定结构域识别囊泡运输相关膜蛋白的胞质尾部信号，为分泌途径中货物分选提供分子基础。
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2. **文献名称**：*SEC24C deficiency disrupts ER export and leads to neurodegenerative phenotypes*
**作者**：Wang, L., et al.
**摘要**：通过敲除细胞模型和小鼠实验，证明SEC24C缺失导致内质网分泌功能异常，影响神经元轴突运输关键蛋白(如APP)的定位，提示SEC24C在神经退行性疾病中可能具有病理意义。
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3. **文献名称**：*High-yield expression and purification of recombinant human SEC24C for functional studies*
**作者**：Chen, J., & Singh, R.
**摘要**：报道了一种利用大肠杆菌系统高效表达并纯化重组人SEC24C蛋白的方法，优化后的蛋白具有体外结合COPII组分及货物蛋白的活性，为后续生化与药物筛选研究提供工具。
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注：以上文献信息为模拟生成，实际研究中请通过PubMed或专业数据库检索具体论文。
SEC24C, a member of the SEC24 family, is a critical component of the COPII-coated vesicles that mediate endoplasmic reticulum (ER)-to-Golgi protein transport in eukaryotic cells. As one of four SEC24 paralogs (SEC24A-D) in humans, SEC24C plays a specialized role in cargo selection by recognizing specific sorting signals on transmembrane proteins during vesicle budding. It forms a heterodimer with SEC23. creating the inner layer of the COPII coat that coordinates cargo packaging and interacts with the outer coat components SEC13/SEC31.
Structurally, SEC24C contains a conserved β-barrel domain for binding SEC23 and an α-solenoid domain that facilitates cargo recognition. Its dysfunction has been linked to developmental disorders and neurological conditions, with studies suggesting its involvement in autophagy regulation and cellular stress responses. Recombinant SEC24C protein, typically produced in mammalian or insect expression systems, serves as a vital tool for studying COPII assembly mechanisms, cargo selectivity, and ER export pathways. Recent research leverages purified SEC24C for structural analyses (e.g., cryo-EM), in vitro COPII reconstitution assays, and screening small molecules targeting vesicular trafficking in cancer and neurodegenerative diseases. Its isoform-specific interactions make it particularly interesting for understanding tissue-selective secretory pathways.
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