| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SF3A2 |
| Uniprot No | Q15428 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-464 aa |
| 活性数据 | MDFQHRPGGK TGSGGVASSS ESNRDRRERL RQLALETIDI NKDPYFMKNH LGSYECKLCL TLHNNEGSYL AHTQGKKHQT NLARRAAKEA KEAPAQPAPE KVKVEVKKFV KIGRPGYKVT KQRDSEMGQQ SLLFQIDYPE IAEGIMPRHR FMSAYEQRIE PPDRRWQYLL MAAEPYETIA FKVPSREIDK AEGKFWTHWN RETKQFFLQF HFKMEKPPAP PSLPAGPPGV KRPPPPLMNG LPPRPPLPES LPPPPPGGLP LPPMPPTGPA PSGPPGPPQL PPPAPGVHPP APVVHPPASG VHPPAPGVHP PAPGVHPPAP GVHPPTSGVH PPAPGVHPPA PGVHPPAPGV HPPAPGVHPP APGVHPPPSA GVHPQAPGVH PAAPAVHPQA PGVHPPAPGM HPQAPGVHPQ PPGVHPSAPG VHPQPPGVHP SNPGVHPPTP MPPMLRPPLP SEGPGNIPPP PPTN |
| 分子量 | 49.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SF3A2蛋白的3篇参考文献概览(文献标题与作者均为虚构，仅供示例)：
1. **"SF3A2 in Spliceosome Assembly: Structural Insights from Recombinant Protein Analysis"**
*作者：Zhang et al. (2020)*
**摘要**：通过重组SF3A2蛋白的体外重构实验，揭示其在剪接体复合物形成中的关键作用，解析了其与其他剪接因子的相互作用界面。
2. **"SF3A2 Overexpression Promotes Cancer Cell Survival via Aberrant Splicing"**
*作者：Kim & Patel (2018)*
**摘要**：研究发现重组SF3A2蛋白在癌细胞中异常高表达，导致选择性剪接错误，进而激活促生存基因通路并增强化疗耐药性。
3. **"CRISPR-Based Screening Identifies SF3A2 as a Regulator of Viral RNA Processing"**
*作者：Wang et al. (2022)*
**摘要**：利用重组SF3A2蛋白进行功能验证，证实其通过调控宿主剪接机器影响多种RNA病毒(如HIV)的基因表达和复制效率。
注：以上文献信息为模拟生成，实际研究中请通过PubMed等数据库检索真实文献。
**Background of Recombinant Human SF3A2 Protein**
The splicing factor 3A subunit 2 (SF3A2) is a critical component of the spliceosome, a macromolecular complex responsible for pre-mRNA splicing in eukaryotic cells. It is part of the SF3a subcomplex, which, along with SF3b and other factors, stabilizes the U2 small nuclear ribonucleoprotein (snRNP) during the assembly of the active spliceosome. SF3A2 plays a key role in recognizing branch site sequences and ensuring accurate intron removal, thereby maintaining RNA splicing fidelity. Dysregulation of SF3A2 has been implicated in splicing errors linked to cancers, neurological disorders, and other diseases.
Recombinant human SF3A2 protein is produced using expression systems (e.g., *E. coli*, mammalian cells) to enable studies on its structure, function, and interactions. This engineered protein often includes affinity tags (e.g., His, GST) for purification and detection. Researchers utilize it to investigate spliceosome assembly mechanisms, screen therapeutic compounds targeting splicing anomalies, or explore disease-related mutations. Its recombinant form provides a standardized tool for elucidating the role of SF3A2 in gene expression regulation and developing biomarkers or therapies for splicing-related pathologies.
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