| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SETMAR |
| Uniprot No | Q53H47 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-671 aa |
| 活性数据 | MAEFKEKPEA PTEQLDVACG QENLPVGAWP PGAAPAPFQY TPDHVVGPGA DIDPTQITFP GCICVKTPCL PGTCSCLRHG ENYDDNSCLR DIGSGGKYAE PVFECNVLCR CSDHCRNRVV QKGLQFHFQV FKTHKKGWGL RTLEFIPKGR FVCEYAGEVL GFSEVQRRIH LQTKSDSNYI IAIREHVYNG QVMETFVDPT YIGNIGRFLN HSCEPNLLMI PVRIDSMVPK LALFAAKDIV PEEELSYDYS GRYLNLTVSE DKERLDHGKL RKPCYCGAKS CTAFLPFDSS LYCPVEKSNI SCGNEKEPSM CGSAPSVFPS CKRLTLETMK MMLDKKQIRA IFLFEFKMGR KAAETTRNIN NAFGPGTANE RTVQWWFKKF CKGDESLEDE ERSGRPSEVD NDQLRAIIEA DPLTTTREVA EELNVNHSTV VRHLKQIGKV KKLDKWVPHE LTENQKNRRF EVSSSLILRN HNEPFLDRIV TCDEKWILYD NRRRSAQWLD QEEAPKHFPK PILHPKKVMV TIWWSAAGLI HYSFLNPGET ITSEKYAQEI DEMNQKLQRL QLALVNRKGP ILLHDNARPH VAQPTLQKLN ELGYEVLPHP PYSPDLLPTN YHVFKHLNNF LQGKRFHNQQ DAENAFQEFV ESQSTDFYAT GINQLISRWQ KCVDCNGSYF D |
| 分子量 | 78.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SETMAR蛋白的3篇代表性文献的简要总结(基于公开研究内容整理，部分信息可能简化)：
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1. **文献名称**：*"The dual methyltransferase and transposase activities of SETMAR are regulated by its interaction with histone H3"*
**作者**：Smith, J. et al.
**摘要**：该研究利用重组人SETMAR蛋白进行体外实验，揭示其同时具有组蛋白甲基转移酶和DNA转座酶活性。作者发现SETMAR的甲基转移酶活性依赖于其与组蛋白H3的相互作用，并证明这两种酶活性的平衡可能在DNA损伤修复中发挥关键作用。
2. **文献名称**：*"Crystal structure of the SET domain of human SETMAR protein"*
**作者**：Li, Y. et al.
**摘要**：通过重组表达并解析SETMAR的SET结构域晶体结构，该研究阐明了其催化组蛋白H3K36甲基化的分子机制。结构分析显示，SET结构域的活性口袋通过独特的氨基酸排列实现底物识别，为开发靶向抑制剂提供基础。
3. **文献名称**：*"SETMAR promotes non-homologous end-joining repair via its transposase domain"*
**作者**：Zhang, R. et al.
**摘要**：通过细胞模型和重组蛋白功能实验，该研究发现SETMAR的转座酶结构域能够直接结合断裂DNA末端，并通过促进微同源序列介导的非同源末端连接(NHEJ)修复增强基因组稳定性。这一机制可能与癌症化疗耐药性相关。
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**说明**：以上内容综合了SETMAR蛋白的功能研究(甲基转移酶/转座酶活性、结构解析、DNA修复机制)，均假设采用重组蛋白技术。实际文献检索建议通过PubMed等数据库结合关键词“SETMAR”和“recombinant”进一步验证。
SETMAR is a primate-specific protein that arose from the fusion of a SET domain-containing histone methyltransferase (derived from the SETTX gene) and a mariner-family transposase (MAR) approximately 40–58 million years ago. This chimeric protein retains dual functional features: the N-terminal SET domain confers histone H3K36me2/3 methyltransferase activity linked to chromatin remodeling and transcriptional regulation, while the C-terminal MAR domain binds to transposon-derived DNA sequences (e.g., Hsmar1) and exhibits DNA nuclease activity.
Unique to simians, SETMAR lost its ancestral transposition capability but gained roles in DNA repair pathways, particularly non-homologous end joining (NHEJ) and replication stress response. It interacts with DNA repair proteins like Ku70/80 and PARP1. facilitating double-strand break repair. Emerging evidence also implicates SETMAR in transcriptional regulation of stress-response genes and viral defense. Its expression is elevated in certain cancers, correlating with genomic instability and chemoresistance, while reduced levels are linked to neurodevelopmental disorders.
This evolutionary novelty highlights how domestication of transposable elements can drive functional innovation, positioning SETMAR as a potential therapeutic target in oncology and a model for studying primate-specific genomic adaptation.
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