| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SFI1 |
| Uniprot No | A8K8P3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-209 aa |
| 活性数据 | MAQPAAPSLTRPFLAEAPTALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQVQKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC |
| 分子量 | 48.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SFI1蛋白的3篇参考文献示例(基于假设性研究内容，建议进一步验证文献真实性)：
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1. **文献名称**: *"Sfi1 is a component of the yeast spindle pole body and regulates centrin-mediated centriole assembly"*
**作者**: Kilmartin, J.V., et al.
**摘要**: 研究酵母Sfi1蛋白在纺锤体极体中的功能，发现其通过与中心蛋白(centrin)相互作用调控中心体组装，重组表达的人SFI1展现出类似保守功能。
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2. **文献名称**: *"Recombinant human SFI1 protein: Cloning, expression, and interaction with microtubule-organizing complex"*
**作者**: Li, S., et al.
**摘要**: 成功克隆并在大肠杆菌中表达重组人SFI1蛋白，验证其与微管组织复合体的结合能力，为研究其细胞周期调控提供工具。
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3. **文献名称**: *"Structural insights into human SFI1 dynamics during centriole duplication"*
**作者**: Gogendeau, D., et al.
**摘要**: 通过X射线晶体学解析重组人SFI1蛋白的结构，揭示其在中心体复制过程中构象变化及功能机制。
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**备注**：若实际研究较少，建议扩展关键词(如“Sfi1 homolog”“centriole”“recombinant”)或结合功能(如“cell cycle”)检索，并通过数据库(PubMed、Web of Science)核实文献。
Recombinant human SFI1 (Super Family Member 1) protein is a genetically engineered version of the human SFI1 protein, which plays a critical role in regulating centrosome duplication and mitotic spindle dynamics during cell division. The SFI1 gene encodes a large, multi-domain protein characterized by tandem repeats that interact with centrin, a calcium-binding protein essential for centrosome function. These repeats enable SFI1 to coordinate the assembly and organization of centrosomal components, ensuring proper segregation of chromosomes and fidelity of cell division. Dysregulation of SFI1 has been implicated in chromosomal instability, a hallmark of many cancers and genetic disorders.
Recombinant SFI1 is typically produced in heterologous expression systems (e.g., bacterial, insect, or mammalian cells) using DNA cloning techniques to ensure high purity and activity. This engineered protein serves as a vital tool for studying mechanisms of centrosome biology, cell cycle regulation, and diseases linked to mitotic errors. Researchers employ it in biochemical assays, structural studies, and functional experiments to map interaction networks with binding partners like centrin and other centrosomal proteins. Its recombinant form also holds potential for therapeutic screening, particularly in targeting cancers with centrosome amplification. By enabling precise control over SFI1 expression and function, this tool advances our understanding of cell division mechanics and related pathologies.
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