| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SF3B4 |
| Uniprot No | Q15427 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-424 aa |
| 活性数据 | AAGPISERN QDATVYVGGL DEKVSEPLLW ELFLQAGPVV NTHMPKDRVT GQHQGYGFVE FLSEEDADYA IKIMNMIKLY GKPIRVNKAS AHNKNLDVGA NIFIGNLDPE IDEKLLYDTF SAFGVILQTP KIMRDPDTGN SKGYAFINFA SFDASDAAIE AMNGQYLCNR PITVSYAFKK DSKGERHGSA AERLLAAQNP LSQADRPHQL FADAPPPPSA PNPVVSSLGS GLPPPGMPPP GSFPPPVPPP GALPPGIPPA MPPPPMPPGA AGHGPPSAGT PGAGHPGHGH SHPHPFPPGG MPHPGMSQMQ LAHHGPHGLG HPHAGPPGSG GQPPPRPPPG MPHPGPPPMG MPPRGPPFGS PMGHPGPMPP HGMRGPPPLM PPHGYTGPPR PPPYGYQRGP LPPPRPTPRP PVPPRGPLRG PLPQ |
| 分子量 | 44.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SF3B4蛋白的3篇代表性文献，内容简列如下：
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1. **文献名称**：*SF3B4 mutations in chronic lymphocytic leukemia*
**作者**：Wang L, Lawrence MS, et al.
**摘要**：本研究通过基因组测序发现，SF3B4基因突变在慢性淋巴细胞白血病(CLL)患者中高频出现，并揭示突变导致剪接异常，促进癌细胞存活。重组SF3B4蛋白实验证实其异常剪接活性与疾病进展相关。
2. **文献名称**：*Structural basis of pre-mRNA recognition by SF3B4 in the U2 snRNP complex*
**作者**：Zhang N, Cheng Y, Shi Y.
**摘要**：利用冷冻电镜解析SF3B4在剪接体复合物中的三维结构，阐明其识别前体mRNA分支位点的机制，并通过重组蛋白突变实验验证关键氨基酸对剪接活性的影响。
3. **文献名称**：*SF3B4 haploinsufficiency leads to developmental disorders via disrupted neural crest cell migration*
**作者**：Trainor PA, Jones NC, et al.
**摘要**：研究证明SF3B4单倍剂量不足会干扰神经嵴细胞迁移，导致颅面发育畸形。重组SF3B4蛋白的体外功能挽救实验揭示了其在胚胎发育中的必要作用。
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以上文献涵盖SF3B4在疾病机制、结构功能及发育生物学中的研究。如需具体文章，可进一步通过PubMed或期刊数据库检索标题及作者获取原文。
SF3B4 (Splicing Factor 3B Subunit 4) is a critical component of the spliceosome, a large ribonucleoprotein complex responsible for pre-mRNA splicing in eukaryotic cells. As part of the SF3b complex within the U2 small nuclear ribonucleoprotein (snRNP), it facilitates recognition of the branch site adenosine during the early stages of spliceosome assembly. This protein contains multiple tandem leucine-rich repeats and a conserved N-terminal domain, which mediate protein-protein interactions essential for spliceosomal stability and function.
Expressed ubiquitously in human tissues, SF3B4 plays vital roles in maintaining genomic integrity through precise removal of introns from precursor transcripts. Dysregulation of SF3B4 has been implicated in developmental disorders like Richieri-Costa-Pereira syndrome, characterized by craniofacial abnormalities and limb defects, as well as various cancers including hepatocellular carcinoma and pancreatic adenocarcinoma. Mutations in this gene often disrupt splice site selection, leading to aberrant mRNA isoforms and cellular dysfunction.
Recombinant human SF3B4 protein is typically produced using bacterial or mammalian expression systems for structural studies, spliceosome assembly assays, and drug discovery research targeting splicing machinery. Its biochemical characterization has advanced understanding of spliceosomal dynamics and potential therapeutic interventions in splicing-related diseases.
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