| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGIP1 |
| Uniprot No | Q9BQI5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-859 aa |
| 活性数据 | MMEGLKKRTRKAFGIRKKEKDTDSTGSPDRDGIQGKKKTQKTQLLLTSFFWLRALSLTLSQKKSNGAPNGFYAEIDWERYNSPELDEEGYSIRPEEPGSTKGKHFYSSSESEEEEESHKKFNIKIKPLQSKDILKNAATVDELKASIGNIALSPSPVRKSPRRSQGAIKRNLSSEEVARPRRSTPTPELISKKPPDDTTALAPLFGPPLESAFDEQKTEVLLDQPEIWGSGQPINPSMESPKLTRPFPTGTPPPLPPKNVPATPPRTGSPLTIGPGNDQSATEVKIEKLPSINDLDSIFGPVLSPKSVAVNAEEKWVHFSDTSPEHVTPELTPREKVVSPPATPDNPADSPAPGPLGPPGPTGPPGPPGPPRNVLSPLNLEEVQKKVAEQTFIKDDYLETISSPKDFGLGQRATPPPPPPPTYRTVVSSPGPGSGPGPGTTSDGKTEAQRYQVICPSLQAGGNELDSYSGASSPARPATPLVPCRSTTPPPPPPRPPSRPKLPPGKPGVGDVSRPFSPPIHSSSPPPIAPLARAESTSSISSTNSLSAATTPTVENEQPSLVWFDRGKFYLTFEGSSRGPSPLTMGAQDTLPVAAAFTETVNAYFEGADPSKCIVKITGEMVLSFPAGITRHFANNPSPAALTFRVINFSRLEHVLPNPQLLCCDNTQNDANTKEFWVNMPNLMTHLKKVSEQKPQATYYNVDMLKYQVSAQGIQSTPLNLAVNWRCEPSSTDLRIDYKYNTDAMTTAVALNNVQFLVPIDGGVTKLQAVLPPAVWNAEQQRILWKIPDISQKSENGGVGSLLARFQLSEGPSKPSPLVVQFTSEGSTLSGCDIELVGAGYRFSLIKKRFAAGKYLADN |
| 分子量 | 119.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人SGIP1蛋白的参考文献示例(部分内容基于类似研究推测，具体文献需根据实际检索验证)：
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1. **文献名称**：*SGIP1 modulates kinetics and interactions of the leptin receptor signaling*
**作者**：Smith A, et al.
**摘要**：本研究利用重组人SGIP1蛋白体外实验，揭示了SGIP1与瘦素受体胞内域的相互作用，通过调控受体二聚化速率影响下游信号传导，可能解释其在肥胖相关代谢紊乱中的调控机制。
2. **文献名称**：*Crystal structure of the SH3 domain of human SGIP1 in complex with endophilin A1*
**作者**：Chen L, et al.
**摘要**：通过重组表达纯化SGIP1的SH3结构域，解析其与内吞关键蛋白Endophilin A1的复合物晶体结构，揭示了SGIP1在内吞过程中调控网格蛋白包被形成的分子机制。
3. **文献名称**：*Functional characterization of SGIP1 in neuronal endocytosis using recombinant protein assays*
**作者**：Wang Y, et al.
**摘要**：该研究在大肠杆菌系统中重组表达SGIP1功能结构域，证明其通过竞争性结合调控Synaptojanin-1磷酸酶活性，从而影响突触囊泡回收效率。
4. **文献名称**：*Development of a mammalian cell-expressed recombinant SGIP1 for therapeutic antibody screening*
**作者**：Kim J, et al.
**摘要**：建立了基于HEK293细胞的重组人SGIP1蛋白表达纯化平台，验证其与抗体的结合活性，为靶向SGIP1的肥胖治疗药物开发提供工具。
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**注意**：以上文献信息为模拟生成，实际需通过PubMed、Web of Science等平台以“SGIP1 recombinant protein”等关键词检索。若研究较少，可扩展至SGIP1互作蛋白(如Endophilin、瘦素受体)的相关文献间接参考。
**Background of Recombinant Human SGIP1 Protein**
SGIP1 (Src Homology 3 Domain Growth Factor Receptor-Bound 2-Like Endophilin Interacting Protein 1) is a cytosolic adaptor protein involved in clathrin-mediated endocytosis and cellular signaling pathways. It interacts with endophilins and other components of the endocytic machinery, playing a regulatory role in receptor internalization, vesicle formation, and membrane trafficking. SGIP1 contains several functional domains, including an N-terminal α-helical region, a mu homology domain (μHD), and a C-terminal SH3-binding motif, enabling its participation in protein-protein interactions.
Studies highlight SGIP1's influence on metabolic homeostasis, particularly in energy balance and insulin signaling. It modulates leptin and insulin receptor trafficking, linking it to obesity and diabetes research. SGIP1 also interacts with huntingtin-associated protein 1 (HAP1), suggesting roles in neurodegenerative disorders like Huntington's disease.
Recombinant human SGIP1 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains functional domains for *in vitro* studies. It is widely used to investigate endocytosis mechanisms, screen therapeutic compounds targeting metabolic or neurological diseases, and analyze protein interaction networks. Its recombinant form enables precise control in biochemical assays, structural studies, and pathway modulation experiments, offering insights into SGIP1's physiological and pathological relevance.
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