| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGKL |
| Uniprot No | Q96BR1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-496 aa |
| 活性数据 | MQRDHTMDYK ESCPSVSIPS SDEHREKKKR FTVYKVLVSV GRSEWFVFRR YAEFDKLYNT LKKQFPAMAL KIPAKRIFGD NFDPDFIKQR RAGLNEFIQN LVRYPELYNH PDVRAFLQMD SPKHQSDPSE DEDERSSQKL HSTSQNINLG PSGNPHAKPT DFDFLKVIGK GSFGKVLLAK RKLDGKFYAV KVLQKKIVLN RKEQKHIMAE RNVLLKNVKH PFLVGLHYSF QTTEKLYFVL DFVNGGELFF HLQRERSFPE HRARFYAAEI ASALGYLHSI KIVYRDLKPE NILLDSVGHV VLTDFGLCKE GIAISDTTTT FCGTPEYLAP EVIRKQPYDN TVDWWCLGAV LYEMLYGLPP FYCRDVAEMY DNILHKPLSL RPGVSLTAWS ILEELLEKDR QNRLGAKEDF LEIQNHPFFE SLSWADLVQK KIPPPFNPNV AGPDDIRNFD TAFTEETVPY SVCVSSDYSI VNASVLEADD AFVGFSYAPP SEDLFL |
| 分子量 | 57.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人 **SGK1**(血清/糖皮质激素调节激酶1)蛋白的参考文献示例(*注：名称“SGKL”可能为笔误，此处假设为SGK1*)：
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1. **文献名称**：*Expression and Characterization of Active Human Serum/Glucocorticoid-Regulated Kinase 1 in Mammalian Cells*
**作者**：Firestone, G.L. et al.
**摘要**：通过哺乳动物细胞表达系统成功制备重组人SGK1蛋白，验证其激酶活性，并研究其在细胞应激反应中对钠离子通道的调控作用。
2. **文献名称**：*Structural Basis for the Activity of the mTOR-Independent SGK1 Inhibitor GSK650394*
**作者**：Sherk, A.B. et al.
**摘要**：利用重组SGK1蛋白进行晶体结构解析，揭示小分子抑制剂GSK650394的作用机制，为糖尿病和癌症治疗提供潜在靶点。
3. **文献名称**：*Recombinant SGK1 rescues ENaC activity in cystic fibrosis airway epithelial cells*
**作者**：Soundararajan, R. et al.
**摘要**：证明重组SGK1蛋白可激活囊性纤维化患者上皮细胞中的上皮钠通道(ENaC)，提示其在改善呼吸道离子失衡中的治疗潜力。
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**注意**：
- 若需求为其他“SGKL”蛋白，请确认名称准确性(如是否为**SGK3**或**SgK223**等同源激酶)。
- 建议通过PubMed或Google Scholar以关键词“recombinant SGK1”检索最新文献。
Serum/glucocorticoid-regulated kinase-like protein (SGKL), also known as Sgk3 or serine/threonine-protein kinase SGK3. is a member of the serine/threonine protein kinase family. It shares structural homology with the serum- and glucocorticoid-regulated kinase (SGK1), featuring a conserved catalytic kinase domain and a unique N-terminal phox homology (PX) domain that mediates phosphoinositide binding. SGKL is primarily regulated by the PI3K/AKT/mTOR signaling pathway and responds to cellular stimuli such as growth factors, hormones, and stress conditions.
Functionally, SGKL plays critical roles in ion transport, cell survival, and proliferation. It regulates sodium reabsorption in renal tubules by modulating the activity of ion channels like Na+/K+-ATPase and epithelial sodium channels (ENaC). SGKL also participates in cellular responses to osmotic stress and maintains electrolyte homeostasis. Unlike SGK1. SGKL exhibits distinct subcellular localization due to its PX domain, which directs it to endosomal and membrane compartments.
Research links SGKL to cancer progression, metabolic disorders, and neurodegenerative diseases. Its dysregulation has been implicated in tumor cell survival under nutrient-poor conditions and chemoresistance. As a recombinant protein, SGKL is used to study kinase signaling mechanisms, screen therapeutic inhibitors, and investigate cellular stress adaptation pathways. Ongoing studies aim to clarify its tissue-specific functions and therapeutic potential in precision medicine.
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