| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGK |
| Uniprot No | O00141 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431 aa |
| 活性数据 | MTVKTEAAKGTLTYSRMRGMVAILIAFMKQRRMGLNDFIQKIANNSYACKHPEVQSILKISQPQEPELMNANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSGPNDLRHFDPEFTEEPVPNSIGKSPDSVLVTASVKEAAEAFLGFSYAPPTDSFL |
| 分子量 | 73.04 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SGK(Serum/Glucocorticoid-regulated Kinase)蛋白的3条参考文献及其摘要内容：
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1. **文献名称**: "Regulation and physiological roles of serum- and glucocorticoid-induced protein kinase (SGK) isoforms"
**作者**: Lang F, et al.
**摘要**: 综述SGK不同亚型(SGK1/2/3)的表达调控机制及生理功能，重点探讨重组SGK1在调节离子通道、细胞增殖及凋亡中的作用，尤其是其在糖尿病和高血压相关代谢调控中的分子机制。
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2. **文献名称**: "Expression, purification, and functional characterization of recombinant human SGK1 protein"
**作者**: Kobayashi T, Deak M, et al.
**摘要**: 报道了通过哺乳动物细胞系统表达并纯化具有激酶活性的重组人SGK1蛋白，验证其磷酸化下游靶标蛋白(如NDRG1)的能力，并证明其活性受PI3K/mTOR信号通路的调控。
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3. **文献名称**: "SGK1 mediates fibrotic responses in diabetic nephropathy"
**作者**: You H, et al.
**摘要**: 研究重组SGK1在糖尿病肾病模型中的作用，发现其通过激活TGF-β/Smad通路促进肾小管上皮细胞纤维化，抑制SGK1表达可减少胶原沉积，提示其作为治疗靶点的潜力。
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以上文献涵盖SGK的分子机制、表达方法及病理功能研究。如需具体年份或补充文献可进一步说明。
Recombinant human serum/glucocorticoid-regulated kinase (SGK) protein is a key signaling molecule involved in cellular stress responses and ion transport regulation. Belonging to the AGC family of serine/threonine kinases, SGK shares structural similarities with Akt/PKB but is distinctively regulated by hormonal and environmental cues. It was initially identified as a glucocorticoid-responsive gene, though later studies showed its activation through aldosterone, osmotic stress, and growth factors via PI3K/mTOR pathways. SGK modulates sodium reabsorption by phosphorylating ion channels (e.g., ENaC) and transporters (e.g., NCC), linking it to blood pressure regulation and electrolyte homeostasis. Its role extends to cell survival, apoptosis, and proliferation by interacting with mTOR, NF-κB, and FOXO transcription factors. Dysregulation of SGK activity is implicated in pathological conditions including hypertension, diabetes, fibrosis, and cancer metastasis. Recombinant SGK proteins are typically produced in E. coli or mammalian expression systems, enabling structural studies and kinase activity assays. These purified tools help investigate SGK’s molecular mechanisms, screen inhibitors, and explore therapeutic targeting. Notably, SGK1 (the most studied isoform) has emerged as a potential drug target for metabolic and inflammatory diseases, though challenges remain in isoform-specific modulation. Recent research also highlights its involvement in immune responses and neurological disorders, broadening its biomedical relevance.
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