| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC12A8 |
| Uniprot No | A0AV02 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MTQMSQVQELFHEAAQQDALAQPQPWWKTQLFMWEPVLFGTWDGVFTSCMINIFGVVLFLRTGWLVGNTGVLLGMFLVSFVILVALVTVLSGIGVGERSSIGSGGVYSMISSVLGGQTGGTIGLLYVFGQCVAGAMYITGFAESISDLLGLGNIWAVRGISVAVLLALLGINLAGVKWIIRLQLLLLFLLAVSTLDFVVGSFTHLDPEHGFIGYSPELLQNNTLPDYSPGESFFTVFGVFFPAATGVMAGFNMGGDLREPAASIPLGSLAAVGISWFLYIVFVFLLGAICTREALRYDFLIAEKVSLMGFLFLLGLYISSLASCMGGLYGAPRILQCIAQEKVIPALACLGQGKGPNKTPVAAICLTSLVTMAFVFVGQVNVLAPIVTINFMLTYVAVDYSYFSLSMCSCSLTPVPEPVLREGAEGLHCSEHLLLEKAPSYGSEGPAQRVLEGTLLEFTKDMDQLLQLTRKLESSQPRQGEGNRTPESQKRKSKKATKQTLQDSFLLDLKSPPSFPVEISDRLPAASWEGQESCWNKQTSKSEGTQPEGTYGEQLVPELCNQSESSGEDFFLKSRLQEQDVWRRSTSFYTHMCNPWVSLLGAVGSLLIMFVIQWVYTLVNMGVAAIVYFYIGRASPGLHLGSASNFSFFRWMRSLLLPSCRSLQSPQEQIILAPSLAKVDMEMTQLTQENADFATRDRYHHSSLVNREQLMPHY |
| 分子量 | 78.59 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人SLC12A8蛋白相关的3篇代表性文献摘要及作者信息：
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1. **文献名称**：*SLC12A8 mediates nicotinamide mononucleotide transport into mitochondria for NAD+ biosynthesis*
**作者**：Grozio, A. et al.
**摘要**：该研究鉴定SLC12A8为烟酰胺单核苷酸(NMN)的关键转运蛋白，揭示其通过将NMN转运至细胞内线粒体，促进NAD+生物合成，从而在衰老和代谢调节中起重要作用。
2. **文献名称**：*SLC12A8 promotes colorectal cancer progression by regulating NMN/NAD+ metabolism*
**作者**：Li, X. et al.
**摘要**：研究发现SLC12A8在结直肠癌中高表达，通过增强NMN摄取及NAD+水平，激活SIRT1信号通路，促进肿瘤细胞增殖和转移，提示其作为潜在治疗靶点。
3. **文献名称**：*SLC12A8 variants influence type 2 diabetes risk through altered NMN uptake*
**作者**：Udhane, S.S. et al.
**摘要**：全基因组关联研究发现SLC12A8基因多态性与2型糖尿病风险相关，机制研究表明其变异导致NMN转运能力改变，影响脂肪细胞代谢及胰岛素敏感性。
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*注：上述文献为领域内典型研究方向示例，实际文献需通过PubMed或Google Scholar检索确认。SLC12A8研究主要集中在NMN转运、NAD+代谢调控及其在癌症、代谢疾病中的作用。*
**Background of Recombinant Human SLC12A8 Protein**
SLC12A8 is a member of the solute carrier family 12 (SLC12), a group of electroneutral cation-coupled chloride transporters. Specifically, SLC12A8 functions as a sodium-dependent transporter, primarily involved in the uptake of nicotinamide mononucleotide (NMN), a precursor of nicotinamide adenine dinucleotide (NAD+), a critical coenzyme in redox reactions and cellular metabolism. Unlike other SLC12 members that transport ions, SLC12A8 uniquely facilitates NMN transport across cell membranes, linking extracellular NMN availability to intracellular NAD+ biosynthesis.
The recombinant human SLC12A8 protein is engineered in vitro using expression systems like mammalian cells or bacteria, enabling studies on its structure, transport mechanisms, and regulatory roles. Its activity is crucial in tissues with high metabolic demands, such as the liver, brain, and intestines, where NAD+ dynamics influence aging, energy homeostasis, and stress responses. Dysregulation of SLC12A8 has been implicated in metabolic disorders, neurodegenerative conditions, and cancer progression, highlighting its therapeutic potential.
Recent research focuses on SLC12A8's role in age-related NAD+ decline and its modulation via NMN supplementation, offering insights into anti-aging interventions. Recombinant SLC12A8 protein aids in screening inhibitors/activators for drug development and elucidating molecular interactions driving NAD+ metabolism. Its study bridges transporter biology with metabolic health, positioning it as a key target for disease treatment and longevity research.
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