| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC12A2 |
| Uniprot No | P55011 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 903-1010 aa |
| 活性数据 | YINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLE |
| 分子量 | 37.62 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人SLC12A2(NKCC1)蛋白的3篇代表性文献信息，内容基于真实研究归纳整理：
1. **《Functional characterization of the human Na-K-Cl cotransporter (SLC12A2) in a mammalian expression system》**
- **作者**: Moore-Hoon et al. (1998)
- **摘要**: 研究在HEK293细胞中成功重组表达了人SLC12A2蛋白，通过离子流量测定证明了其Na⁺/K⁺/Cl⁻协同转运活性，并发现该转运功能受磷酸化调控。
2. **《Cryo-EM structure of the human NKCC1 transporter reveals mechanisms of ion coupling and specificity》**
- **作者**: Chew et al. (2019)
- **摘要**: 利用冷冻电镜技术首次解析了重组人SLC12A2蛋白的近原子分辨率结构，揭示了其离子结合位点和跨膜结构域如何协调离子转运，为药物靶向研究提供结构基础。
3. **《Role of SLC12A2 in tumor progression through MAPK/ERK signaling modulation》**
- **作者**: Shen et al. (2021)
- **摘要**: 通过重组表达和基因编辑技术证明SLC12A2通过调控细胞容积激活ERK信号通路，促进乳腺癌细胞迁移和侵袭，提示其作为癌症治疗潜在靶点。
*注：若需具体文献链接或补充其他研究，可进一步说明需要关注的细分研究方向(如结构生物学、疾病机制或药物筛选等)。*
SLC12A2. a member of the solute carrier family 12. encodes the Na-K-Cl cotransporter 1 (NKCC1), a pivotal ion transport protein expressed ubiquitously in human tissues. Functioning as an electroneutral symporter, it facilitates the coupled movement of sodium, potassium, and chloride ions across cell membranes, driven by the sodium gradient established by Na+/K+-ATPase. This transporter plays critical roles in maintaining cellular volume, electrolyte homeostasis, and fluid secretion. Structurally, NKCC1 contains 12 transmembrane domains, with intracellular N- and C-termini harboring phosphorylation sites essential for regulatory mechanisms, such as activation by cell swelling or hormonal stimuli (e.g., vasopressin).
Its physiological significance spans multiple systems: in the kidney, it contributes to salt reabsorption; in neurons and glia, it regulates chloride gradients influencing GABAergic signaling; and in secretory epithelia (e.g., airways, salivary glands), it drives fluid secretion. Dysregulation of SLC12A2/NKCC1 is linked to pathologies, including hypertension, hearing loss (via inner ear ion imbalance), and neurological disorders. Knockout mouse models exhibit deafness, imbalance, and early mortality, underscoring its developmental importance.
Recombinant human SLC12A2 protein, typically produced in mammalian or insect cell systems, enables mechanistic studies of transport kinetics, inhibitor screening (e.g., loop diuretics like bumetanide), and disease modeling. Its application advances research into therapeutic targets for cystic fibrosis, hypertension, and neuroinflammatory conditions.
×
