| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC20A1 |
| Uniprot No | Q8WUM9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-679 aa |
| 活性数据 | MATLITSTTAATAASGPLVDYLWMLILGFIIAFVLAFSVGANDVANSFGTAVGSGVVTLKQACILASIFETVGSVLLGAKVSETIRKGLIDVEMYNSTQGLLMAGSVSAMFGSAVWQLVASFLKLPISGTHCIVGATIGFSLVAKGQEGVKWSELIKIVMSWFVSPLLSGIMSGILFFLVRAFILHKADPVPNGLRALPVFYACTVGINLFSIMYTGAPLLGFDKLPLWGTILISVGCAVFCALIVWFFVCPRMKRKIEREIKCSPSESPLMEKKNSLKEDHEETKLSVGDIENKHPVSEVGPATVPLQAVVEERTVSFKLGDLEEAPERERLPSVDLKEETSIDSTVNGAVQLPNGNLVQFSQAVSNQINSSGHYQYHTVHKDSGLYKELLHKLHLAKVGDCMGDSGDKPLRRNNSYTSYTMAICGMPLDSFRAKEGEQKGEEMEKLTWPNADSKKRIRMDSYTSYCNAVSDLHSASEIDMSVKAEMGLGDRKGSNGSLEEWYDQDKPEVSLLFQFLQILTACFGSFAHGGNDVSNAIGPLVALYLVYDTGDVSSKVATPIWLLLYGGVGICVGLWVWGRRVIQTMGKDLTPITPSSGFSIELASALTVVIASNIGLPISTTHCKVGSVVSVGWLRSKKAVDWRLFRNIFMAWFVTVPISGVISAAIMAIFRYVILRM |
| 分子量 | 100.43 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC20A1(PiT-1)蛋白的参考文献摘要整理，供参考：
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1. **文献名称**: "Functional characterization of recombinant human SLC20A1/PiT-1 phosphate transporter"
**作者**: Fujioka et al. (2018)
**摘要**: 研究通过昆虫细胞表达系统成功重组表达了人SLC20A1蛋白，验证其钠依赖的磷酸盐转运活性，并揭示了胞外结构域在底物识别中的关键作用。
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2. **文献名称**: "SLC20A1 serves as a receptor for retrovirus infection in humans"
**作者**: Beckstead et al. (2015)
**摘要**: 发现重组表达的SLC20A1是哺乳动物逆转录病毒(如GALV)的功能性受体，揭示了其跨膜结构域与病毒表面糖蛋白的相互作用机制。
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3. **文献名称**: "Structural insights into phosphate transport by human PiT-1/SLC20A1"
**作者**: O’Hara & Palsson (2020)
**摘要**: 通过冷冻电镜技术解析重组人SLC20A1的三维结构，阐明了其底物结合口袋及钠离子协同运输的构象变化，为设计靶向药物提供结构基础。
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4. **文献名称**: "Impaired phosphate transport in SLC20A1 knockout cells rescued by recombinant expression"
**作者**: Campos-Barros & Terkelsen (2016)
**摘要**: 利用基因编辑构建SLC20A1敲除细胞系，证实重组蛋白可恢复细胞磷酸盐摄取能力，提示其在遗传性磷酸盐代谢紊乱中的潜在治疗价值。
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注：以上文献为虚拟示例，实际引用时需以具体数据库(如PubMed、Web of Science)检索结果为准，建议结合关键词**“SLC20A1 recombinant expression”或“PiT-1 functional studies”**深入检索最新研究。
SLC20A1. also known as PiT-1. is a member of the solute carrier family 20 (SLC20), which comprises sodium-dependent phosphate transporters. This ubiquitously expressed protein plays a critical role in inorganic phosphate (Pi) homeostasis, facilitating cellular Pi uptake through Na⁺-coupled co-transport. Structurally, it contains 12 transmembrane domains with intracellular N- and C-termini, featuring conserved glycosylation sites in extracellular loops that influence its trafficking and stability.
Originally identified as the receptor for gibbon ape leukemia virus (GALV) and related retroviruses, SLC20A1 supports both physiological processes and pathogenic entry. It contributes to bone mineralization, cellular metabolism, and signal transduction by maintaining phosphate balance. Dysregulation is linked to vascular calcification, kidney diseases, and tumor progression, where elevated expression in cancer cells may promote proliferation through phosphate-dependent pathways.
Recombinant human SLC20A1 proteins are typically produced in heterologous systems (e.g., HEK293 cells) for functional studies. These tools enable research into phosphate transport mechanisms, structure-function relationships, and therapeutic targeting. Its dual role as a nutrient transporter and viral receptor makes SLC20A1 a unique subject for studying metabolic disorders, viral pathogenesis, and potential therapeutic interventions, particularly in diseases associated with phosphate dysregulation or retroviral infections.
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