| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC20A2 |
| Uniprot No | Q08357 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-652 aa |
| 活性数据 | MAMDEYLWMVILGFIIAFILAFSVGANDVANSFGTAVGSGVVTLRQACILASIFETTGSVLLGAKVGETIRKGIIDVNLYNETVETLMAGEVSAMVGSAVWQLIASFLRLPISGTHCIVGSTIGFSLVAIGTKGVQWMELVKIVASWFISPLLSGFMSGLLFVLIRIFILKKEDPVPNGLRALPVFYAATIAINVFSIMYTGAPVLGLVLPMWAIALISFGVALLFAFFVWLFVCPWMRRKITGKLQKEGALSRVSDESLSKVQEAESPVFKELPGAKANDDSTIPLTGAAGETLGTSEGTSAGSHPRAAYGRALSMTHGSVKSPISNGTFGFDGHTRSDGHVYHTVHKDSGLYKDLLHKIHIDRGPEEKPAQESNYRLLRRNNSYTCYTAAICGLPVHATFRAADSSAPEDSEKLVGDTVSYSKKRLRYDSYSSYCNAVAEAEIEAEEGGVEMKLASELADPDQPREDPAEEEKEEKDAPEVHLLFHFLQVLTACFGSFAHGGNDVSNAIGPLVALWLIYKQGGVTQEAATPVWLLFYGGVGICTGLWVWGRRVIQTMGKDLTPITPSSGFTIELASAFTVVIASNIGLPVSTTHCKVGSVVAVGWIRSRKAVDWRLFRNIFVAWFVTVPVAGLFSAAVMALLMYGILPYV |
| 分子量 | 96.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC20A2蛋白研究的3篇代表性文献示例，内容基于该蛋白的功能及疾病关联研究整理而成：
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1. **文献名称**：*"Mutations in SLC20A2 impair phosphate transport and underlie familial idiopathic basal ganglia calcification"*
**作者**：Wang C, et al.
**摘要内容**：该研究鉴定了SLC20A2基因突变与家族性基底 ganglia 钙化的关联，通过重组表达突变体蛋白，发现其磷酸盐转运能力显著降低，提示蛋白功能缺陷导致病理性钙沉积。
2. **文献名称**：*"Functional characterization of SLC20A2 variants in primary familial brain calcification using a novel mammalian cell model"*
**作者**：Jensen N, et al.
**摘要内容**：作者构建了重组人SLC20A2蛋白的哺乳动物细胞表达系统，分析了多种致病突变对磷酸盐摄取的影响，揭示了不同突变导致功能丧失的分子机制。
3. **文献名称**：*"Structural insights into human SLC20A2/PiT2 phosphate transporter by cryo-EM"*
**作者**：Lee S, et al.
**摘要内容**：本研究通过冷冻电镜解析了重组SLC20A2蛋白的三维结构，阐明了其钠离子依赖的磷酸盐转运机制，为靶向药物设计提供结构基础。
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**备注**：以上文献为示例，实际研究需通过学术数据库(如PubMed)检索具体标题及作者。如需真实文献，建议使用关键词“SLC20A2 recombinant”“PiT2 expression”进一步筛选。
SLC20A2. a member of the solute carrier family 20. is a sodium-dependent phosphate transporter crucial for cellular phosphate homeostasis. Encoded by the SLC20A2 gene, this ubiquitously expressed protein facilitates inorganic phosphate (Pi) uptake across cell membranes by coupling it with sodium ions via a secondary active transport mechanism. Structurally, it contains 12 transmembrane domains and belongs to the type III Na+/Pi cotransporter group. Beyond its role in nutrient transport, SLC20A2 participates in diverse physiological processes, including cellular signaling, bone mineralization, and vascular function. Notably, mutations in SLC20A2 are strongly linked to primary familial brain calcification (PFBC), a neurological disorder characterized by abnormal calcium deposits in brain regions, leading to motor impairments and cognitive decline. The pathogenic mechanism likely involves disrupted phosphate metabolism, triggering ectopic calcification. Recombinant human SLC20A2 proteins are generated using expression systems (e.g., bacterial, mammalian) to study its biochemical properties, substrate specificity, and mutation-induced functional alterations. These recombinant tools aid in elucidating PFBC pathophysiology and developing therapeutic strategies. Research also explores its potential interactions with viral entry pathways, expanding its relevance to infectious diseases. As a druggable target, SLC20A2 continues to attract attention for both neurological disorders and phosphate-related metabolic imbalances.
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