| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC2A4 |
| Uniprot No | P14672 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-509 aa |
| 活性数据 | MPSGFQQIGSEDGEPPQQRVTGTLVLAVFSAVLGSLQFGYNIGVINAPQKVIEQSYNETWLGRQGPEGPSSIPPGTLTTLWALSVAIFSVGGMISSFLIGIISQWLGRKRAMLVNNVLAVLGGSLMGLANAAASYEMLILGRFLIGAYSGLTSGLVPMYVGEIAPTHLRGALGTLNQLAIVIGILIAQVLGLESLLGTASLWPLLLGLTVLPALLQLVLLPFCPESPRYLYIIQNLEGPARKSLKRLTGWADVSGVLAELKDEKRKLERERPLSLLQLLGSRTHRQPLIIAVVLQLSQQLSGINAVFYYSTSIFETAGVGQPAYATIGAGVVNTVFTLVSVLLVERAGRRTLHLLGLAGMCGCAILMTVALLLLERVPAMSYVSIVAIFGFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGFSNWTSNFIIGMGFQYVAEAMGPYVFLLFAVLLLGFFIFTFLRVPETRGRTFDQISAAFHRTPSLLEQEVKPSTELEYLGPDEND |
| 分子量 | 81.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC2A4(GLUT4)蛋白的3篇参考文献摘要示例：
1. **文献名称**: "Insulin-regulated trafficking of GLUT4 requires ubiquitination"
**作者**: S. Lalioti et al.
**摘要**: 研究揭示了GLUT4在脂肪细胞中的转运机制，发现其细胞内循环依赖于泛素化修饰，重组GLUT4表达模型被用于验证胰岛素刺激下蛋白膜定位的动态变化。
2. **文献名称**: "Structural insights into human GLUT4 by cryo-EM"
**作者**: N. Fan et al.
**摘要**: 通过冷冻电镜技术解析重组人SLC2A4蛋白的三维结构，阐明了其底物结合位点和转运葡萄糖的构象变化，为糖尿病药物设计提供结构基础。
3. **文献名称**: "Functional expression of GLUT4 in Saccharomyces cerevisiae"
**作者**: A. Leto et al.
**摘要**: 报道在酵母系统中成功重组表达人SLC2A4蛋白，验证其葡萄糖摄取功能，证实该模型可用于高通量筛选调节葡萄糖代谢的化合物。
(注：以上为虚拟文献示例，实际文献需通过PubMed/Google Scholar等数据库检索确认。)
The solute carrier family 2 member 4 (SLC2A4), commonly known as glucose transporter type 4 (GLUT4), is a pivotal insulin-regulated facilitative glucose transporter encoded by the SLC2A4 gene. Predominantly expressed in adipose tissue and skeletal muscle, GLUT4 plays a central role in maintaining systemic glucose homeostasis by mediating insulin-stimulated glucose uptake. Under basal conditions, GLUT4 resides in intracellular vesicles; upon insulin signaling, these vesicles translocate to the cell membrane, enhancing glucose absorption to regulate postprandial blood sugar levels. Structurally, GLUT4 contains 12 transmembrane domains with cytoplasmic N- and C-termini, a hallmark of the SLC2A transporter family. Dysregulation of GLUT4 expression, trafficking, or function is strongly linked to insulin resistance and type 2 diabetes. Recombinant human SLC2A4 protein, produced via genetic engineering in systems like mammalian or bacterial cells, serves as a critical tool for studying glucose metabolism disorders, drug discovery, and therapeutic strategies targeting insulin resistance. Its applications extend to elucidating molecular mechanisms of vesicle trafficking, insulin signaling pathways, and glucose transport dynamics in metabolic diseases.
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