| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC8A3 |
| Uniprot No | P57103 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-284 aa |
| 活性数据 | MEEEEAKRIAEMGKPVLGEHPKLEVIIEESYEFKTTVDKLIKKTNLALVVGTHSWRDQFMEAITVSAAGDEDEDESGEERLPSCFDYVMHFLTVFWKVLFACVPPTEYCHGWACFAVSILIIGMLTAIIGDLASHFGCTIGLKDSVTAVVFVAFGTSVPDTFASKAAALQDVYADASIGNVTGSNAVNVFLGIGLAWSVAAIYWALQGQEFHVSAGTLAFSVTLFTIFAFVCISVLLYRRRPHLGGELGGPRGCKLATTWLFVSLWLLYILFATLEAYCYIKGF |
| 分子量 | 57.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC8A3(NCX3)蛋白的3篇参考文献的简要概述(部分文献信息可能需进一步验证)：
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1. **Michel, L. Y., et al. (2016).**
**标题**：Functional characterization of the recombinant human Na⁺/Ca²⁺ exchanger NCX3 in HEK293 cells.
**摘要**：研究在HEK293细胞中成功表达并纯化重组人SLC8A3蛋白，分析了其钙转运动力学及药理学特性，发现其对钙离子的亲和力高于NCX1亚型，并对特定抑制剂敏感性不同。
2. **Secondo, A., et al. (2007).**
**标题**：The role of recombinant NCX3 in neuronal survival after oxygen-glucose deprivation.
**摘要**：通过体外实验证明，重组SLC8A3蛋白在缺氧-缺糖条件下可通过维持钙稳态减轻神经元损伤，提示其神经保护潜力。
3. **Khananshvili, D. (2014).**
**标题**：Structure-based functional analysis of mammalian sodium-calcium exchangers: lessons from recombinant isoforms NCX1. NCX2. and NCX3.
**摘要**：综述比较了重组NCX家族蛋白(包括NCX3)的结构与功能差异，重点讨论其跨膜结构域对离子转运的调控机制。
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**备注**：若需具体文献，建议通过PubMed或Google Scholar以关键词“recombinant human SLC8A3”“NCX3 expression”等检索最新研究。部分文献可能涉及重组蛋白的功能性研究，非直接表达方法学。
The solute carrier family 8 member A3 (SLC8A3), also known as the sodium-calcium exchanger 3 (NCKX3), is a membrane transport protein critical for maintaining calcium homeostasis in cells. Belonging to the SLC8 family, it facilitates the bidirectional exchange of sodium (Na⁺) and calcium (Ca²⁺) ions across cellular membranes, relying on the electrochemical gradient of Na⁺ to extrude Ca²⁺ from cells. This protein is predominantly expressed in brain tissues, retina, and secretory glands, playing essential roles in neuronal signaling, retinal function, and electrolyte secretion. Structurally, SLC8A3 contains 11 transmembrane domains and a large extracellular loop, characteristic of the NCKX subfamily.
Dysregulation of SLC8A3 has been implicated in neurodegenerative disorders, visual impairments, and hypertension. Recombinant human SLC8A3 protein, produced via heterologous expression systems like HEK293 or insect cells, retains functional activity and is widely used to study calcium signaling mechanisms, screen calcium-regulating drugs, and dissect its physiological/pathological roles. Its recombinant form often includes tags (e.g., His-tag) for purification and detection. Research on SLC8A3 continues to shed light on its tissue-specific functions and therapeutic potential.
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