| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC9A1 |
| Uniprot No | P19634 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MVLRSGICGLSPHRIFPSLLVVVALVGLLPVLRSHGLQLSPTASTIRSSEPPRERSIGDVTTAPPEVTPESRPVNHSVTDHGMKPRKAFPVLGIDYTHVRTPFEISLWILLACLMKIGFHVIPTISSIVPESCLLIVVGLLVGGLIKGVGETPPFLQSDVFFLFLLPPIILDAGYFLPLRQFTENLGTILIFAVVGTLWNAFFLGGLMYAVCLVGGEQINNIGLLDNLLFGSIISAVDPVAVLAVFEEIHINELLHILVFGESLLNDAVTVVLYHLFEEFANYEHVGIVDIFLGFLSFFVVALGGVLVGVVYGVIAAFTSRFTSHIRVIEPLFVFLYSYMAYLSAELFHLSGIMALIASGVVMRPYVEANISHKSHTTIKYFLKMWSSVSETLIFIFLGVSTVAGSHHWNWTFVISTLLFCLIARVLGVLGLTWFINKFRIVKLTPKDQFIIAYGGLRGAIAFSLGYLLDKKHFPMCDLFLTAIITVIFFTVFVQVLGQGRAGPCLGDPHRLFPWKERKACDLKCDSSPSSTTNLLCDLGRATPPFWASVSSIVK |
| 分子量 | 61.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC9A1(NHE1)蛋白的3篇参考文献示例及摘要概括：
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1. **文献名称**：*Expression and Functional Characterization of the Human Na+/H+ Exchanger NHE1 in HEK293 Cells*
**作者**：Wakabayashi, S., et al.
**摘要**：该研究描述了在HEK293细胞中重组表达人SLC9A1(NHE1)蛋白的方法，并通过同位素示踪实验验证其Na+/H+交换活性，揭示了其在细胞pH稳态中的关键作用及对细胞增殖的影响。
2. **文献名称**：*Purification and Structural Insights into the Human NHE1 Protein*
**作者**：Hisamitsu, T., et al.
**摘要**：本文报道了重组人SLC9A1蛋白的高效纯化流程，利用冷冻电镜技术解析其跨膜结构域的三维构象，为理解其离子转运机制及药物靶向设计提供了结构基础。
3. **文献名称**：*Regulation of NHE1 by Phosphorylation in Cancer Cell Migration*
**作者**：Putney, L.K., et al.
**摘要**：研究通过重组表达SLC9A1蛋白，结合突变体分析发现其C端磷酸化位点对肿瘤细胞迁移的调控作用，揭示了该蛋白在癌症转移中的潜在治疗价值。
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注：上述文献为示例，实际引用时建议通过PubMed或Web of Science检索真实发表的论文。
**Background of Recombinant Human SLC9A1 Protein**
The solute carrier family 9 member A1 (SLC9A1), also known as Na⁺/H⁺ exchanger 1 (NHE1), is a ubiquitously expressed transmembrane protein that regulates intracellular pH and sodium homeostasis. It facilitates the electroneutral exchange of intracellular protons (H⁺) for extracellular sodium ions (Na⁺), critical for maintaining cell volume, proliferation, and survival. SLC9A1 is composed of 12 membrane-spanning domains and a cytoplasmic C-terminal tail, which mediates regulatory interactions with kinases, scaffolding proteins, and cytoskeletal components. Its activity is dynamically modulated by osmotic stress, growth factors, hormones, and acidosis.
Dysregulation of SLC9A1 is linked to pathologies such as cancer metastasis, cardiovascular diseases, and neurological disorders, where aberrant pH regulation promotes disease progression. Recombinant human SLC9A1 protein is commonly produced in heterologous expression systems (e.g., mammalian, insect, or bacterial cells) for structural and functional studies. It serves as a tool to investigate molecular mechanisms of ion transport, screen therapeutic inhibitors, or model diseases. Engineering recombinant SLC9A1 often includes tags (e.g., His, GFP) for purification or tracking, enabling advanced research into its role in cellular physiology and drug discovery targeting pH-dependent pathways.
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