| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SP4 |
| Uniprot No | Q02446 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-784 aa |
| 活性数据 | MSDQKKEEEEEAAAAAAMATEGGKTSEPENNNKKPKTSGSQDSQPSPLALLAATCSKIGTPGENQATGQQQIIIDPSQGLVQLQNQPQQLELVTTQLAGNAWQLVASTPPASKENNVSQPASSSSSSSSSNNGSASPTKTKSGNSSTPGQFQVIQVQNPSGSVQYQVIPQLQTVEGQQIQINPTSSSSLQDLQGQIQLISAGNNQAILTAANRTASGNILAQNLANQTVPVQIRPGVSIPLQLQTLPGTQAQVVTTLPINIGGVTLALPVINNVAAGGGTGQVGQPAATADSGTSNGNQLVSTPTNTTTSASTMPESPSSSTTCTTTASTSLTSSDTLVSSADTGQYASTSASSSERTIEESQTPAATESEAQSSSQLQPNGMQNAQDQSNSLQQVQIVGQPILQQIQIQQPQQQIIQAIPPQSFQLQSGQTIQTIQQQPLQNVQLQAVNPTQVLIRAPTLTPSGQISWQTVQVQNIQSLSNLQVQNAGLSQQLTITPVSSSGGTTLAQIAPVAVAGAPITLNTAQLASVPNLQTVSVANLGAAGVQVQGVPVTITSVAGQQQGQDGVKVQQATIAPVTVAVGGIANATIGAVSPDQLTQVHLQQGQQTSDQEVQPGKRLRRVACSCPNCREGEGRGSNEPGKKKQHICHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGELDSSVTEVLGSPRIVTVAAISQDSNPATPNVSTNMEEF |
| 分子量 | 112.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SP4蛋白的3篇典型文献摘要概览：
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1. **文献名称**: *"Structural characterization of human transcription factor SP4 and its involvement in cellular proliferation"*
**作者**: Smith A., et al.
**摘要**: 研究解析了重组人SP4蛋白的晶体结构，发现其锌指结构域对DNA结合具有关键作用，并证实SP4通过调控细胞周期相关基因影响肿瘤细胞增殖。
2. **文献名称**: *"Functional analysis of SP4 in neuronal development using recombinant protein models"*
**作者**: Chen L., et al.
**摘要**: 通过重组SP4蛋白体外实验，揭示了其在神经元分化和突触形成中的作用，表明SP4异常表达可能导致神经发育障碍(如自闭症)。
3. **文献名称**: *"SP4-mediated transcriptional regulation in schizophrenia-associated pathways"*
**作者**: Kim H., et al.
**摘要**: 利用重组SP4蛋白进行染色质免疫沉淀分析，发现SP4与精神分裂症风险基因(如*DRD2*)的启动子区域结合，提示其参与疾病相关的表观遗传调控。
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注：上述文献为虚拟示例，实际研究中建议通过PubMed或Web of Science检索最新成果。若需具体论文，可补充说明研究方向(如结构、疾病关联等)。
**Background of Recombinant Human SP4 Protein**
SP4 (Specificity Protein 4) is a member of the SP/KLF transcription factor family, characterized by a conserved DNA-binding domain containing three Cys2-His2 zinc fingers. It regulates gene expression by binding to GC-rich promoter regions, influencing cellular processes like proliferation, differentiation, and apoptosis. SP4 is particularly abundant in the brain, where it plays critical roles in neuronal development, synaptic plasticity, and dopaminergic signaling.
Genetic studies link SP4 to neuropsychiatric disorders, including schizophrenia, bipolar disorder, and autism spectrum disorders, with identified risk-associated SNPs in the SP4 gene. Reduced SP4 expression in animal models leads to behavioral and neurodevelopmental deficits, underscoring its functional importance.
Recombinant human SP4 protein is produced using prokaryotic (e.g., *E. coli*) or eukaryotic expression systems, ensuring proper post-translational modifications. Purification via affinity tags (e.g., His-tag) allows high-purity yields. This recombinant protein serves as a vital tool for studying SP4’s DNA-binding properties, protein interactions, and regulatory mechanisms. It also aids in drug discovery, enabling screens for compounds modulating SP4 activity. Additionally, it is used in diagnostic research to explore SP4’s role as a biomarker in neurological diseases.
Overall, recombinant SP4 protein bridges molecular insights into transcription regulation and its implications in health and disease.
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