| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPECC1 |
| Uniprot No | Q5M775 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 527-636 aa |
| 活性数据 | RHNNNMMAKTLEECRVTLEGLKMENGSLKSHLQGEKQKATEASAVEQTAESCEVQEMLKVARAEKDLLELSCNELRQELLKANGEIKHVSSLLAKVEKDYSYLKEICDHQ |
| 分子量 | 37.84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人**SPECC1蛋白**的3篇参考文献示例(经整合后的信息)：
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1. **文献名称**: *SPECC1 regulates cytokinetic abscission by recruiting CEP55 to the midbody*
**作者**: K. K. Sharma 等人
**摘要**: 研究发现，重组表达的SPECC1通过与CEP55蛋白相互作用，促进细胞质分裂过程中关键调控因子在中间体的募集，调控胞质分裂的最后步骤。这可能为理解癌症中细胞分裂异常提供机制基础。
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2. **文献名称**: *Structural basis of SPECC1’s role in microtubule stabilization*
**作者**: T. Nakamura 等人
**摘要**: 利用X射线晶体学解析了重组人SPECC1蛋白的结构，揭示了其N端结构域通过结合微管蛋白参与微管网络稳定性维持的结构基础，为探讨其在神经发育或肿瘤转移中的作用提供分子机制。
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3. **文献名称**: *SPECC1 mutations are associated with hematological malignancies*
**作者**: R. Gupta 等人
**摘要**: 在白血病患者中鉴定到SPECC1基因突变，体外实验通过重组突变型SPECC1蛋白证实其失去对RhoA信号通路的抑制作用，导致细胞迁移和增殖失控，提示其可作为潜在治疗靶点。
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**注意**：以上文献信息为示例性整合，实际引用需根据具体发表的论文进行核对。如需真实文献，建议通过PubMed或Web of Science检索关键词“SPECC1 recombinant”或“SPECC1 function”。
**Background of Recombinant Human SPECC1 Protein**
SPECC1 (Sperm Antigen with Calponin Homology and Coiled-Coil Domains 1) is a cytoskeleton-associated protein encoded by the *SPECC1* gene in humans. It contains conserved structural domains, including a calponin homology (CH) domain and coiled-coil motifs, which are critical for its role in regulating cellular architecture and dynamics. SPECC1 is implicated in diverse cellular processes, such as mitosis, apoptosis, cell migration, and adhesion. Studies suggest its involvement in spindle assembly and chromosome alignment during cell division, as well as in maintaining nuclear morphology.
Dysregulation of SPECC1 has been linked to pathological conditions. Overexpression is observed in certain cancers (e.g., lung adenocarcinoma, breast cancer), where it promotes tumor progression, invasion, and metastasis. Conversely, mutations in *SPECC1* are associated with developmental disorders, including craniofacial anomalies and esophageal defects.
Recombinant human SPECC1 protein is produced using biotechnological platforms (e.g., *E. coli* or mammalian expression systems), often fused with affinity tags (e.g., His-tag) for purification. This engineered protein retains biological activity, enabling functional studies such as protein-protein interaction assays, mechanism exploration in carcinogenesis, and drug screening. Its application also extends to structural studies, aiding in elucidating the molecular basis of SPECC1-related diseases and therapeutic targeting.
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