| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPDYC |
| Uniprot No | Q5MJ68 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-293aa |
| 活性数据 | MLWAIPELGS PCPISISYEM SDSQDPTTSP VVTTQVELGG CSRQGGGNGF LRFRQHQEVQ AFLSLLEDSF VQEFLSKDPC FQISDKYLLA MVLVYFQRAH LKLSEYTHSS LFLALYLAND MEEDLEGPKC EIFPWALGKD WCLRVGKFLH QRDKLWARMG FRAVVSRQCC EEVMAKEPFH WAWTRDRRPH HGGVQRVCPQ VPVRLPRGPG LSPPHCSPCG LPQHCSSHLL KPVSSKCPSL TSECHRPPSQ NYLSRVKNAW GGDFLIVLPP QMQLEPGTYS LRIFPKPPAR PGH |
| 分子量 | 31.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SPDYC蛋白的3篇代表性文献概述(示例基于领域相关研究整合，非真实文献)：
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1. **文献名称**: *SPDYC interacts with Cyclin-dependent kinase 2 (CDK2) in germ cell development*
**作者**: Smith J, et al.
**摘要**: 研究揭示了SPDYC蛋白在哺乳动物生殖细胞中的关键作用，通过重组表达技术证实其与CDK2的相互作用，调控减数分裂进程，缺失会导致精子发生异常。
2. **文献名称**: *Recombinant SPDYC production in E. coli: Structural insights and functional characterization*
**作者**: Li X, et al.
**摘要**: 报道了在大肠杆菌中高效表达和纯化重组人SPDYC蛋白的方法，通过X射线晶体学解析其三维结构，并验证其在体外的磷酸酶活性调控机制。
3. **文献名称**: *SPDYC as a novel oncogenic co-factor in MYC-driven cancers*
**作者**: Garcia-R, et al.
**摘要**: 利用重组SPDYC蛋白进行功能实验，发现其在MYC信号通路中增强肿瘤细胞增殖，提示其可能作为癌症治疗的潜在靶点。
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**注**：上述文献为假设性示例，实际研究中建议通过PubMed或Web of Science以“SPDYC recombinant”或“SPDYC protein function”为关键词检索最新文献。
**Background of Recombinant Human SPDYC Protein**
SPDYC (SPD-1 homolog, Y-linked, and cytoplasmically localized) is a member of the SPDY (Speedy) protein family, which plays critical roles in cell cycle regulation, particularly during the G1/S and G2/M transitions. Originally identified for its role in oocyte maturation in *Xenopus*, SPDYC shares structural similarities with Speedy/Ringo proteins, known for binding and activating cyclin-dependent kinases (CDKs) independent of cyclins. Unlike its paralog SPDYA (SpeedyA), SPDYC lacks a nuclear localization signal, leading to cytoplasmic localization and distinct regulatory functions in mitotic progression and cell proliferation.
The human *SPDYC* gene, located on chromosome 10. encodes a protein implicated in diverse biological processes, including germ cell development, apoptosis, and cancer. Dysregulation of SPDYC has been linked to hepatocellular carcinoma, testicular germ cell tumors, and other malignancies, suggesting its potential as a therapeutic target. Recombinant SPDYC protein is typically produced using bacterial (e.g., *E. coli*) or eukaryotic expression systems, enabling studies on its interactions with CDKs (e.g., CDK1/CDK2), post-translational modifications, and role in signaling pathways.
As a research tool, recombinant SPDYC facilitates investigations into cell cycle mechanics, reproductive biology, and oncogenesis, offering insights for drug development targeting cell cycle-related disorders.
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