| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPERT |
| Uniprot No | Q8NA61 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-448aa |
| 活性数据 | MSPLECSECF GDQLLHRTYT WQLTLHSRPN YTRKRDTRSE SLEIPISVVL PQRGTAEPFP RLHNLYSTPR CAQQAALPRL SRRMASQHSY PLNRFSSVPL DPMERPMSQA DLELDYNPPR VQLSDEMFVF QDGRWVNENC RLQSPYFSPS ASFHHKLHHK RLAKECMLQE ENKSLREENK ALREENRMLS KENKILQVFW EEHKASLGRE ESRAPSPLLH KDSASLEVVK KDHVALQVPR GKEDSTLQLL REENRALQQL LEQKQAYWAQ AEDTAAPAEE SKPAPSPHEE PCSPGLLQDQ GSGLSSRFEE PKGPPARQED SKELRALRKM VSNMSGPSGE EEAKVGPGLP DGCQPLQLLR EMRQALQALL KENRLLQEEN RTLQVLRAEH RGFQEENKAL WENNKLKLQQ KLVIDTVTEV TARMEMLIEE LYAFMPARSQ DPKKPSRV |
| 分子量 | 51.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人SPERT蛋白的参考文献及其简要内容(基于近年研究虚构，实际文献需自行验证)：
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1. **文献名称**：*SPERT regulates cAMP signaling in human testicular somatic cells*
**作者**：Chen L, et al.
**摘要**：研究揭示了重组人SPERT蛋白通过调控cAMP/PKA信号通路，在睾丸支持细胞的增殖和激素分泌中起关键作用，可能影响精子发生过程。
2. **文献名称**：*Recombinant human SPERT facilitates Hedgehog pathway activation in vitro*
**作者**：Wang Y, Smith J.
**摘要**：报道了重组SPERT蛋白通过结合Patched受体增强Hedgehog信号通路的活性，为癌症和发育障碍相关研究提供新靶点。
3. **文献名称**：*Structural and functional characterization of human SPERT in germ cell development*
**作者**：Kumar R, et al.
**摘要**：通过X射线晶体学解析SPERT蛋白结构，证明其通过结合微管相关蛋白参与生殖细胞减数分裂的时空调控。
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**注意**：以上为示例性内容，实际文献需在PubMed或Web of Science等平台以 **"SPERT protein"、“spermatogenesis-related protein”** 等关键词检索确认。若研究较少，可扩展至相关家族蛋白(如ESX1、PATE等)或生殖发育调控领域。
SPERT (short proline-rich enhancer protein), also known as PC4 and SFRS1-interacting protein (PSIP1), is a chromatin-associated protein involved in transcriptional regulation and DNA repair. It was initially identified through its interaction with PC4. a transcriptional coactivator, and later found to bind splicing factors like SFRS1. SPERT contains a C-terminal PHD finger domain, which mediates histone binding, and an N-terminal region rich in proline and serine residues.
This protein plays a dual role in modulating transcription elongation and RNA splicing. Studies suggest SPERT acts as a scaffold, bridging transcriptional machinery and chromatin modifiers to influence gene expression. It also participates in maintaining genome stability by facilitating DNA damage response pathways. Dysregulation of SPERT has been linked to cancers, including prostate and colorectal cancers, where altered expression correlates with tumor progression.
Recombinant human SPERT protein (rhSPERT) is engineered using eukaryotic expression systems to preserve post-translational modifications. It serves as a critical tool for in vitro studies exploring transcriptional mechanisms, chromatin dynamics, and cancer biology. Ongoing research aims to elucidate its structural interactions and therapeutic potential in targeting SPERT-associated pathways.
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