| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPRR1B |
| Uniprot No | P22528 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-89aa |
| 活性数据 | MSSQQQKQPC TPPPQLQQQQ VKQPCQPPPQ EPCIPKTKEP CHPKVPEPCH PKVPEPCQPK VPEPCHPKVP EPCPSIVTPA PAQQKTKQK |
| 分子量 | 9.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SPRR1B蛋白的3篇代表性文献(虚构示例，供参考)：
1. **文献名称**：*SPRR1B as a novel biomarker in epithelial barrier dysfunction*
**作者**：Marshall, R. et al.
**摘要**：研究揭示了SPRR1B蛋白在皮肤屏障形成中的关键作用，发现其通过参与角质包膜交联增强表皮机械强度，并在银屑病模型中表达异常。
2. **文献名称**：*Pro-inflammatory cytokine regulation of SPRR1B in airway epithelial cells*
**作者**：Hsu, C. et al.
**摘要**：报道了IL-4/IL-13通过STAT6信号通路上调气道上皮细胞中SPRR1B的表达，提示其在过敏性炎症中的潜在调控机制。
3. **文献名称**：*SPRR1B promotes tumor metastasis via EGFR signaling in squamous cell carcinoma*
**作者**：Yin, L. et al.
**摘要**：发现SPRR1B通过激活EGFR通路增强鳞状细胞癌的侵袭性，提示其作为癌症治疗靶点的可能性。
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注：以上文献及内容为模拟，实际研究需通过PubMed/Google Scholar检索确认。
The small proline-rich protein 1B (SPRR1B) is a member of the SPRR family, primarily expressed in keratinocytes during terminal differentiation of the epidermis. It plays a critical role in forming the cornified envelope, a protective barrier that ensures mechanical resilience and environmental resistance in stratified squamous epithelia. SPRR1B is enriched in proline and cysteine residues, enabling cross-linking with other structural proteins like loricrin, involucrin, and filaggrin through transglutaminase-mediated bonds. This contributes to skin barrier integrity, hydration, and defense against pathogens or oxidative stress.
Recombinant human SPRR1B protein is produced using heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its applications include investigating epidermal differentiation pathways, skin disorders (e.g., psoriasis, atopic dermatitis), and epithelial stress responses. Emerging research links SPRR1B dysregulation to pathological conditions, including squamous cell carcinoma and chronic wounds, highlighting its dual role in tissue repair and cancer progression. Additionally, recombinant SPRR1B aids in developing biomimetic models for drug testing or regenerative therapies targeting barrier-deficient diseases. Further studies aim to elucidate its post-translational modifications and interactions in dynamic cellular microenvironments.
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