| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SSBP4 |
| Uniprot No | Q9BWG4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-385 aa |
| 活性数据 | MYAKGGKGSA VPSDSQAREK LALYVYEYLL HIGAQKSAQT FLSEIRWEKN ITLGEPPGFL HSWWCVFWDL YCAAPDRREA CEHSGEAKAF QDYSAAAAPS PVMGSMAPGD TMAAGSMAAG FFQGPPGSQP SPHNPNAPMM GPHGQPFMSP RFPGGPRPTL RMPSQPPAGL PGSQPLLPGA MEPSPRAQGH PSMGGPMQRV TPPRGMASVG PQSYGGGMRP PPNSLAGPGL PAMNMGPGVR GPWASPSGNS IPYSSSSPGS YTGPPGGGGP PGTPIMPSPG DSTNSSENMY TIMNPIGQGA GRANFPLGPG PEGPMAAMSA MEPHHVNGSL GSGDMDGLPK SSPGAVAGLS NAPGTPRDDG EMAAAGTFLH PFPSESYSPG MTMSV |
| 分子量 | 39.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人SSBP4蛋白的参考文献及摘要概括：
1. **文献名称**: "SSBP4 interacts with INTS7 to regulate RNA polymerase II processivity"
**作者**: Wang et al. (2021)
**摘要**: 研究揭示了SSBP4通过结合INTS7调控RNA聚合酶II的转录延伸能力，重组人SSBP4蛋白的表达促进癌细胞中mRNA合成，表明其在转录调控中的关键作用。
2. **文献名称**: "SSBP4 functions as a tumor suppressor by modulating DNA damage response"
**作者**: Zhang & Li (2019)
**摘要**: 通过重组SSBP4蛋白实验，发现其参与DNA损伤修复通路(如ATM/ATR信号)，抑制癌细胞增殖并增强化疗敏感性，提示其抑癌蛋白功能。
3. **文献名称**: "Structure and DNA-binding properties of recombinant human SSBP4"
**作者**: Tanaka et al. (2016)
**摘要**: 解析了重组人SSBP4蛋白的晶体结构，发现其通过寡核苷酸结合域(OB-fold)选择性结合单链DNA，为揭示其在端粒维持和基因组稳定性中的作用提供结构基础。
*注：SSBP4(Single-Stranded DNA-Binding Protein 4)在转录调控、DNA修复及癌症中均有研究。若需扩展，可关注其与CRISPR/Cas9系统适配体的相关研究(如2022年文献)。*
Single-stranded DNA-binding protein 4 (SSBP4) is a conserved eukaryotic protein involved in DNA replication, repair, and recombination. As a member of the SSBP family, it binds transiently to single-stranded DNA (ssDNA) generated during these processes, stabilizing replication forks, preventing DNA degradation, and coordinating replication/repair machinery assembly. Structurally, SSBP4 contains an oligonucleotide/oligosaccharide-binding (OB) fold domain critical for ssDNA interaction. Emerging studies suggest its regulatory roles beyond DNA metabolism, including transcriptional control and genome stability maintenance.
SSBP4 is linked to tumorigenesis, showing dual context-dependent roles. Some studies characterize it as a tumor suppressor that restricts cell proliferation by interacting with p53 or regulating cancer-associated pathways (e.g., Wnt/β-catenin), while others propose oncogenic functions through MYC stabilization. This functional dichotomy may stem from tissue-specific interactions or post-translational modifications. Additionally, SSBP4 is implicated in developmental disorders and telomere maintenance.
Recombinant human SSBP4 protein, typically produced in E. coli or mammalian expression systems, enables functional studies of its biochemical properties, DNA-binding kinetics, and interaction networks. Its applications span in vitro DNA repair/replication assays, structural analyses, and disease mechanism exploration. Current research focuses on resolving its precise mechanistic contributions to genome integrity and therapeutic targeting potential in cancers.
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