| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SSPN |
| Uniprot No | Q14714 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-217 aa |
| 活性数据 | MEPKKGTGAPKECGEEEPRTCCGCRFPLLLALLQLALGIAVTVVGFLMASISSSLLVRDTPFWAGIIVCLVAYLGLFMLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAVAFAAHHYSQLTQFTCETTLDSCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVCGLVCLLACFVMWKHRYQVFYVGVRICSLTASEGPQQKI |
| 分子量 | 49.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SSPN蛋白的3-4篇文献参考(基于公开信息整理，可能需要根据实际数据库补充完整信息)：
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1. **文献名称**：*"Cloning and characterization of the human sarcospan gene and its promoter region"*
**作者**：Croissant C, et al.
**摘要**：该研究克隆了人类SSPN基因并分析了其启动子区域，探讨了SSPN在细胞膜骨架中的结构与功能，为重组蛋白表达提供了基础序列信息。
2. **文献名称**：*"Sarcospan regulates integrin activation and signaling in dystrophic muscle"*
**作者**：Kemaladewi DU, et al.
**摘要**：通过重组SSPN蛋白实验，揭示SSPN在调节肌营养不良模型中整合素信号通路的作用，为靶向治疗提供了分子机制依据。
3. **文献名称**：*"Recombinant SSPN improves muscle function in a murine model of Duchenne muscular dystrophy"*
**作者**：Nelson DM, et al.
**摘要**：研究利用重组SSPN蛋白干预杜氏肌营养不良小鼠模型，发现其可部分恢复肌肉细胞膜稳定性并缓解病理症状。
4. **文献名称**：*"Functional characterization of SSPN in cell adhesion via recombinant protein assays"*
**作者**：Zhou Y, et al.
**摘要**：通过体外重组SSPN蛋白实验，证明SSPN通过与层粘连蛋白相互作用增强细胞粘附能力，提示其潜在临床应用价值。
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**注**：以上为模拟参考文献，实际文献名称和内容需根据具体数据库(如PubMed、Web of Science)检索确认。建议以关键词“recombinant SSPN protein”或“sarcospan expression”查阅最新研究。
Sarcospan (SSPN) is a 25-kDa transmembrane protein integral to the dystrophin-glycoprotein complex (DGC), which stabilizes the muscle cell membrane during contraction. It is expressed in skeletal, cardiac, and smooth muscle, as well as in non-muscle tissues like the brain and retina. Structurally, SSPN contains four transmembrane domains and interacts with other DGC components, including dystroglycans and sarcoglycans, to reinforce the cell membrane and facilitate signaling pathways. Its role in maintaining membrane integrity is critical for protecting muscles from mechanical stress.
Recombinant human SSPN protein is engineered using expression systems (e.g., bacteria, mammalian cells) to produce purified, bioactive forms for research. Its production enables studies on DGC assembly, muscle physiology, and diseases like muscular dystrophies, where SSPN deficiency exacerbates pathology. Beyond muscular disorders, SSPN has emerging roles in cell adhesion, cancer metastasis, and cellular signaling, linked to integrin-mediated pathways. Recombinant SSPN is also explored for therapeutic applications, including gene therapy and protein replacement strategies to restore DGC function. Current research focuses on optimizing its stability and delivery to harness its potential in treating neuromuscular conditions and related pathologies.
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