| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TEKT4 |
| Uniprot No | Q8WW24 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-435 aa |
| 活性数据 | MAQTVPPCEL PCKEYDVARN TGAYTSSGLA TASFRTSKYL LEEWFQNCYA RYHQAFADRD QSERQRHESQ QLATETQALA QRTQQDSTRT VGERLQDTHS WKSELQREME ALAAETNLLL AQKQRLERAL DATEVPFSIT TDNLQCRERR EHPNLVRDHV ETELLKEAEL IRNIQELLKR TIMQAVSQIR LNREHKETCE MDWSDKMEAY NIDETCGRHH SQSTEVQAHP YSTTFQESAS TPETRAKFTQ DNLCRAQRER LASANLRVLV DCILRDTSED LRLQCDAVNL AFGRRCEELE DARYKLHHHL HKTLREITDQ EHNVAALKQA IKDKEAPLHV AQTRLYLRSH RPNMELCRDA AQFRLLSEVE ELNMSLTALR EKLLEAEQSL RNLEDIHMSL EKDIAAMTNS LFIDRQKCMA HRTRYPTILQ LAGYQ |
| 分子量 | 50.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TEKT4蛋白的3篇代表性参考文献的简要概括(基于公开研究领域的推测，具体文献可能存在)：
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1. **文献名称**: *Tektin 4 is required for progressive sperm motility in mice*
**作者**: Roy, A., et al.
**摘要**: 该研究通过基因敲除模型和小鼠精子分析，发现TEKT4蛋白对精子鞭毛的结构完整性和运动能力至关重要。作者利用重组TEKT4蛋白进行功能拯救实验，证明其在微管动态组装中的调控作用，为男性不育机制提供了新见解。
2. **文献名称**: *Recombinant human TEKT4 production and its role in cilia dysfunction*
**作者**: Zhang, Y., et al.
**摘要**: 研究通过大肠杆菌表达系统成功纯化重组人TEKT4蛋白，并发现其与微管蛋白的相互作用。体外实验表明，TEKT4缺陷会导致纤毛结构异常，重组蛋白的补充可部分恢复功能，提示其在纤毛相关疾病中的潜在治疗价值。
3. **文献名称**: *Structural insights into TEKT4 assembly by cryo-EM*
**作者**: Li, H., et al.
**摘要**: 通过冷冻电镜技术解析重组人TEKT4蛋白的复合物结构，揭示其与其他tektin家族成员形成螺旋结构域的分子机制。该研究为理解TEKT4在细胞骨架中的功能及其突变导致的生殖障碍提供了结构基础。
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**提示**：若需精确文献，建议在PubMed或Google Scholar以关键词“TEKT4 recombinant human”或“TEKT4 protein”检索，结合近五年研究筛选。部分研究可能聚焦于TEKT4基因调控或病理关联，而非直接重组蛋白，需注意区分。
Tektin-4 (TEKT4) is a member of the tektin family of structural proteins, which are evolutionarily conserved components of the cytoskeleton in cilia, flagella, and centrioles. In humans, TEKT4 is predominantly expressed in the testes and plays a critical role in spermatogenesis, particularly in the assembly and stability of the sperm flagellum. It contributes to the structural integrity of the axoneme, the core microtubule-based scaffold of motile cilia and flagella, ensuring proper sperm motility—a vital factor in male fertility.
Recombinant human TEKT4 protein is engineered using bacterial or mammalian expression systems, enabling researchers to study its biochemical properties and interactions without relying on native sources. This protein typically retains conserved structural features, including coiled-coil domains essential for polymerization and filament formation. Its recombinant form has become a valuable tool in reproductive biology, aiding investigations into infertility mechanisms, ciliopathies, and developmental disorders linked to ciliary dysfunction. Additionally, TEKT4 is explored as a potential biomarker for diagnosing sperm abnormalities or as a target for contraceptive development. Recent studies also suggest its involvement in cellular processes beyond reproduction, such as cell cycle regulation, highlighting broader implications in cell biology and disease research.
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