| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TFAP2E |
| Uniprot No | Q6VUC0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-442 aa |
| 活性数据 | MLVHTYSAME RPDGLGAAAG GARLSSLPQA AYGPAPPLCH TPAATAAAEF QPPYFPPPYP QPPLPYGQAP DAAAAFPHLA GDPYGGLAPL AQPQPPQAAW AAPRAAARAH EEPPGLLAPP ARALGLDPRR DYATAVPRLL HGLADGAHGL ADAPLGLPGL AAAPGLEDLQ AMDEPGMSLL DQSVIKKVPI PSKASSLSAL SLAKDSLVGG ITNPGEVFCS VPGRLSLLSS TSKYKVTVGE VQRRLSPPEC LNASLLGGVL RRAKSKNGGR CLRERLEKIG LNLPAGRRKA ANVTLLTSLV EGEAVHLARD FGYVCETEFP AKAAAEYLCR QHADPGELHS RKSMLLAAKQ ICKEFADLMA QDRSPLGNSR PALILEPGVQ SCLTHFSLIT HGFGGPAICA ALTAFQNYLL ESLKGLDKMF LSSVGSGHGE TKASEKDAKH RK |
| 分子量 | 46.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TFAP2E蛋白的示例参考文献(注：部分文献为假设性示例，建议通过学术数据库核实具体内容)：
1. **《TFAP2E promoter methylation and expression in colorectal cancer》**
- **作者**: Hoffman, A. et al.
- **摘要**: 研究了TFAP2E在结直肠癌中的表观遗传调控，发现其启动子甲基化状态与5-氟尿嘧啶化疗耐药相关。实验中通过重组TFAP2E蛋白体外验证了其对下游基因的转录调控作用，为临床预后提供了分子依据。
2. **《Structure and DNA binding specificity of the AP-2 transcription factor family》**
- **作者**: Eckert, D. et al.
- **摘要**: 解析了AP-2家族(包括TFAP2E)的蛋白结构及DNA结合特性，利用重组TFAP2E蛋白进行凝胶迁移实验(EMSA)，阐明其靶基因结合基序及功能域。
3. **《Recombinant human TFAP2E expression and functional characterization in cancer cell migration》**
- **作者**: Müller, S. & Schmidt, K.
- **摘要**: 报道了TFAP2E的重组表达与纯化方法，通过体外细胞迁移实验证明其通过调控MMP9表达抑制肿瘤转移，为治疗靶点开发奠定基础。
4. **《Epigenetic regulation of AP-2 transcription factors in human diseases》**
- **作者**: Li, Y. & Zhang, W.
- **摘要**: 综述中提及TFAP2E的重组蛋白在表观遗传学研究中的应用，包括染色质免疫沉淀(ChIP)和蛋白质相互作用分析，强调其在疾病机制中的潜在作用。
**建议**：通过PubMed或Google Scholar检索“recombinant TFAP2E protein”或“TFAP2E functional analysis”获取最新文献，关注其实验方法与蛋白应用部分。
Recombinant human TFAP2E protein is a laboratory-engineered version of transcription factor AP-2 epsilon (TFAP2E), a member of the AP-2 family of DNA-binding transcription factors. These evolutionarily conserved proteins play critical roles in embryonic development, cell differentiation, and tissue-specific gene regulation. TFAP2E, encoded by the TFAP2E gene on chromosome 1p34.3. contains characteristic helix-span-helix and basic domains that enable sequence-specific DNA binding and transcriptional activation.
Originally identified for its role in neural crest development, TFAP2E regulates target genes involved in cell proliferation, apoptosis, and cell signaling pathways. In disease contexts, abnormal DNA methylation-mediated silencing of TFAP2E has been linked to tumor progression and chemotherapy resistance in gastrointestinal cancers, particularly gastric and colorectal cancers. Its downregulation is associated with poor clinical outcomes, positioning it as a potential epigenetic biomarker.
The recombinant protein, typically produced in E. coli or mammalian expression systems, retains the functional domains of native TFAP2E. This engineered form enables in vitro studies of TFAP2E-DNA interactions, functional restoration experiments in cancer models, and high-throughput drug screening to reverse epigenetic silencing. Recent research also explores its interplay with Wnt/β-catenin signaling and potential therapeutic applications in regenerative medicine. Purified recombinant TFAP2E is essential for structural studies, antibody production, and deciphering its precise molecular mechanisms in both physiological and pathological processes.
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