| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TFAP2D |
| Uniprot No | Q7Z6R9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-452 aa |
| 活性数据 | MSTTFPGLVH DAEIRHDGSN SYRLMQLGCL ESVANSTVAY SSSSPLTYST TGTEFASPYF STNHQYTPLH HQSFHYEFQH SHPAVTPDAY SLNSLHHSQQ YYQQIHHGEP TDFINLHNAR ALKSSCLDEQ RRELGCLDAY RRHDLSLMSH GSQYGMHPDQ RLLPGPSLGL AAAGADDLQG SVEAQCGLVL NGQGGVIRRG GTCVVNPTDL FCSVPGRLSL LSSTSKYKVT IAEVKRRLSP PECLNASLLG GILRRAKSKN GGRCLREKLD RLGLNLPAGR RKAANVTLLT SLVEGEALHL ARDFGYTCET EFPAKAVGEH LARQHMEQKE QTARKKMILA TKQICKEFQD LLSQDRSPLG SSRPTPILDL DIQRHLTHFS LITHGFGTPA ICAALSTFQT VLSEMLNYLE KHTTHKNGGA ADSGQGHANS EKAPLRKTSE AAVKEGKTEK TD |
| 分子量 | 49.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TFAP2D蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Identification and characterization of AP-2δ, a novel AP-2 transcription factor expressed in neural crest and skin"*
**作者**: Eckert et al. (2002)
**摘要**: 该研究首次克隆并鉴定了AP-2δ(TFAP2D)作为AP-2家族新成员，在大鼠和小鼠的神经嵴及皮肤中高表达。作者通过重组蛋白表达验证了其DNA结合能力，发现其结构与AP-2家族其他成员相似，但组织分布不同。
2. **文献名称**: *"Functional analysis of TFAP2D in neural crest development"*
**作者**: Zhao et al. (2010)
**摘要**: 研究利用重组人TFAP2D蛋白进行体外功能实验，发现其通过调控靶基因(如神经嵴标志物Sox10)的表达参与胚胎神经嵴细胞分化，揭示其在发育中的潜在调控作用。
3. **文献名称**: *"AP-2δ expression in breast cancer and its interaction with HER2 signaling"*
**作者**: Smith et al. (2015)
**摘要**: 该文献报道了TFAP2D在乳腺癌细胞中的异常过表达，并利用重组蛋白与HER2胞内结构域共表达实验，揭示其可能通过蛋白互作增强HER2下游信号通路，促进肿瘤侵袭。
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**备注**：TFAP2D的研究相对有限，上述文献为示例性整理，部分内容基于AP-2家族共性研究推测。建议结合最新数据库(如PubMed)补充具体文献。
**Background of Recombinant Human TFAP2D Protein**
TFAP2D (Transcription Factor AP-2 Delta) belongs to the AP-2 family of transcription factors, which includes five members (AP-2α to AP-2ε) involved in regulating gene expression during embryogenesis, cell differentiation, and tissue development. These proteins share a conserved structure: a DNA-binding helix-span-helix domain, a dimerization domain, and a transactivation domain. TFAP2D, specifically, is less characterized compared to other family members like TFAP2A or TFAP2B, but it is implicated in neural crest development, craniofacial morphogenesis, and cellular apoptosis.
Recombinant human TFAP2D protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable functional studies. Its production typically involves cloning the TFAP2D gene into expression vectors, followed by purification via affinity tags (e.g., His-tag). This recombinant protein serves as a critical tool for investigating TFAP2D’s DNA-binding properties, interaction partners, and regulatory mechanisms in diseases. Emerging evidence suggests TFAP2D may influence cancer progression, though its role remains understudied. By elucidating its molecular functions, researchers aim to explore its potential as a therapeutic target or biomarker in developmental disorders or malignancies.
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