| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TFAP4 |
| Uniprot No | Q01664 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-338 aa |
| 活性数据 | MEYFMVPTQKVPSLQHFRKTEKEVIGGLCSLANIPLTPETQRDQERRIRREIANSNERRRMQSINAGFQSLKTLIPHTDGEKLSKAAILQQTAEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSPDIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIAQQVQLQQQQEQVRLLHQEKLEREQQQLRTQLLPPPAPTHHPTVIVPAPPPPPSHHINVVTMGPSSVINSVSTSRQNLDTIVQAIQHIEGTQEKQELEEEQRRAVIVKPVRSCPEAPTSDTASDSEASDSDAMDQSREEPSGDGELP |
| 分子量 | 65.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人TFAP4(Transcription Factor AP-4)蛋白的文献摘要信息：
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1. **文献名称**: *"AP-4: A redox-sensitive transcriptional activator of the human glutathione S-transferase P1 gene"*
**作者**: Xie, T., et al.
**摘要**: 本研究揭示了重组TFAP4蛋白通过氧化还原敏感机制调控谷胱甘肽-S-转移酶P1(GSTP1)的转录，并参与细胞抗氧化应激反应的功能机制。
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2. **文献名称**: *"TFAP4 promotes hepatocellular carcinoma growth and invasion via mTORC1 signaling"*
**作者**: Wang, Y., et al.
**摘要**: 研究发现重组TFAP4蛋白通过激活mTORC1信号通路，促进肝癌细胞的增殖、侵袭和转移，提示其作为潜在癌症治疗靶点。
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3. **文献名称**: *"Structural and functional analysis of AP-4 transcription factor DNA-binding domain"*
**作者**: Kim, J., & Dynan, W.S.
**摘要**: 该文献解析了重组TFAP4蛋白的DNA结合域结构，揭示了其特异性识别靶基因启动子区域的分子基础，为研究其转录调控机制提供了结构生物学证据。
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(注：以上文献为模拟示例，实际研究中请通过PubMed或Google Scholar检索最新文献。)
Here’s a concise overview of recombinant human TFAP4 protein:
Transcription Factor AP-4 (TFAP4), also known as AP-4. is a member of the basic helix-loop-helix leucine zipper (bHLH-ZIP) family of transcription factors. It binds to E-box DNA sequences (CAGCTG) and regulates the expression of genes involved in cell cycle progression, differentiation, and apoptosis. TFAP4 plays critical roles in embryonic development, tissue homeostasis, and cancer biology, where it is frequently overexpressed and associated with tumor proliferation, metastasis, and poor prognosis.
Recombinant human TFAP4 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research applications. This enables studies of its DNA-binding properties, protein-protein interactions, and regulatory mechanisms. Researchers utilize it to investigate TFAP4's role in oncogenesis, particularly its crosstalk with pathways like Wnt/β-catenin and p53. as well as its potential as a therapeutic target. Its recombinant form is essential for structural analysis, inhibitor screening, and mechanistic studies in cancer biology and developmental disorders.
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