| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF20 |
| Uniprot No | P17024 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-532 aa |
| 活性数据 | MMFQDSVAFE DVAVSFTQEE WALLDPSQKN LYRDVMQETF KNLTSVGKTW KVQNIEDEYK NPRRNLSLMR EKLCESKESH HCGESFNQIA DDMLNRKTLP GITPCESSVC GEVGTGHSSL NTHIRADTGH KSSEYQEYGE NPYRNKECKK AFSYLDSFQS HDKACTKEKP YDGKECTETF ISHSCIQRHR VMHSGDGPYK CKFCGKAFYF LNLCLIHERI HTGVKPYKCK QCGKAFTRST TLPVHERTHT GVNADECKEC GNAFSFPSEI RRHKRSHTGE KPYECKQCGK VFISFSSIQY HKMTHTGEKP YECKQCGKAF RCGSHLQKHG RTHTGEKPYE CRQCGKAFRC TSDLQRHEKT HTEDKPYGCK QCGKGFRCAS QLQIHERTHS GEKPHECKEC GKVFKYFSSL RIHERTHTGE KPHECKQCGK AFRYFSSLHI HERTHTGDKP YECKVCGKAF TCSSSIRYHE RTHTGEKPYE CKHCGKAFIS NYIRYHERTH TGEKPYQCKQ CGKAFIRASS CREHERTHTI NR |
| 分子量 | 61.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前可公开检索的生物医学文献中关于人ZNF20蛋白的直接研究较为有限，以下为根据锌指蛋白家族常见研究模式模拟的参考文献格式，供参考：
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1. **文献名称**: "Cloning and Expression Analysis of Human ZNF20 Gene"
**作者**: Zhang L, et al.
**摘要**: 本研究成功克隆了人ZNF20基因的cDNA序列，构建了原核表达载体并在大肠杆菌中诱导表达。通过His标签纯化获得了重组ZNF20蛋白，Western blot验证其正确性，为后续功能研究奠定基础。
2. **文献名称**: "ZNF20 Protein Binds to GC-rich DNA Motifs in vitro"
**作者**: Kim S, Park JH.
**摘要**: 利用重组表达的ZNF20蛋白进行凝胶迁移实验(EMSA)，发现其特异性结合GC富集区的DNA序列，提示ZNF20可能通过转录调控参与基因表达。
3. **文献名称**: "Subcellular Localization and Post-translational Modification of ZNF20"
**作者**: Müller R, et al.
**摘要**: 通过荧光标记技术证实ZNF20主要定位于细胞核，并在哺乳动物细胞中实现重组表达。质谱分析发现其存在磷酸化修饰，可能影响DNA结合活性。
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**说明**：以上文献为模拟示例，实际研究建议通过PubMed或Google Scholar以“ZNF20 recombinant protein”“ZNF20 gene function”为关键词查询最新文献。部分锌指蛋白功能可能存在共性文献，可参考拓展。
Zinc finger protein 20 (ZNF20), a member of the zinc finger protein family, is characterized by its conserved C2H2-type zinc finger domains, which enable sequence-specific DNA binding and participation in transcriptional regulation. This nuclear protein is encoded by the *ZNF20* gene located on human chromosome 19 and is implicated in diverse biological processes, including cell differentiation, proliferation, and epigenetic modulation. Structurally, ZNF20 contains multiple tandem zinc finger motifs and a Kruppel-associated box (KRAB) domain, a common feature in transcriptional repressors that recruit chromatin-modifying complexes to silence gene expression.
Recombinant human ZNF20 protein is typically produced using expression systems like *E. coli* or mammalian cell cultures, often fused with tags (e.g., His-tag) for purification and detection. Its recombinant form facilitates functional studies to elucidate DNA-binding specificity, protein-protein interactions, and regulatory roles in gene networks. Emerging evidence links ZNF20 to hematopoiesis and cancer biology, with studies suggesting its involvement in leukemogenesis through aberrant transcriptional repression. Additionally, ZNF20 may interact with chromatin remodelers like histone deacetylases (HDACs), influencing chromatin structure and gene accessibility. Despite progress, its precise physiological targets and mechanisms remain partially unresolved, necessitating further research. Recombinant ZNF20 is a valuable tool for dissecting zinc finger protein functions and exploring therapeutic strategies targeting transcriptional dysregulation in diseases.
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