| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF394 |
| Uniprot No | Q53GI3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-561 aa |
| 活性数据 | MNSSLTAQRR GSDAELGPWV MAARSKDAAP SQRDGLLPVK VEEDSPGSWE PNYPAASPDP ETSRLHFRQL RYQEVAGPEE ALSRLRELCR RWLRPELLSK EQILELLVLE QFLTILPEEL QAWVREHCPE SGEEAVAVVR ALQRALDGTS SQGMVTFEDT AVSLTWEEWE RLDPARRDFC RESAQKDSGS TVPPSLESRV ENKELIPMQQ ILEEAEPQGQ LQEAFQGKRP LFSKCGSTHE DRVEKQSGDP LPLKLENSPE AEGLNSISDV NKNGSIEGED SKNNELQNSA RCSNLVLCQH IPKAERPTDS EEHGNKCKQS FHMVTWHVLK PHKSDSGDSF HHSSLFETQR QLHEERPYKC GNCGKSFKQR SDLFRHQRIH TGEKPYGCQE CGKSFSQSAA LTKHQRTHTG EKPYTCLKCG ERFRQNSHLN RHQSTHSRDK HFKCEECGET CHISNLFRHQ RLHKGERPYK CEECEKSFKQ RSDLFKHHRI HTGEKPYGCS VCGKRFNQSA TLIKHQRIHT GEKPYKCLEC GERFRQSTHL IRHQRIHQNK VLSAGRGGSR L |
| 分子量 | 64.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性参考文献示例，供您参考。由于ZNF394研究可能较局限，建议通过学术数据库(如PubMed、Google Scholar)检索最新文献：
1. **文献名称**：*Cloning and Functional Characterization of Human ZNF394 as a Transcriptional Repressor*
**作者**：Zhang L, et al.
**摘要**：本研究成功克隆并原核表达了重组人ZNF394蛋白，体外实验表明其通过锌指结构域特异性结合DNA启动子区域，抑制靶基因(如肿瘤相关基因)的转录活性。
2. **文献名称**：*ZNF394 Promotes Cell Proliferation in Hepatocellular Carcinoma via Modulation of the MAPK Pathway*
**作者**：Chen X, et al.
**摘要**：通过重组ZNF394蛋白与癌细胞共培养实验，发现其通过激活MAPK信号通路增强肝癌细胞增殖，提示其在癌症进展中的潜在作用。
3. **文献名称**：*Structural Insights into the DNA-Binding Motif of ZNF394 Revealed by X-ray Crystallography*
**作者**：Wang Y, et al.
**摘要**：解析重组ZNF394蛋白的晶体结构，揭示其锌指结构域的三维构象及DNA结合关键残基，为靶向设计抑制剂提供结构基础。
4. **文献名称**：*ZNF394 Interacts with Histone Deacetylases to Regulate Epigenetic Silencing*
**作者**：Kim S, et al.
**摘要**：利用重组ZNF394进行pull-down实验，证实其与HDAC1/2结合形成复合物，协同参与基因启动子的组蛋白去乙酰化及表观遗传调控。
**备注**：以上为模拟案例，实际文献需结合具体研究方向检索确认。若需真实文献，建议使用关键词“ZNF394 recombinant”或“ZNF394 protein function”查询数据库。
Zinc finger protein 394 (ZNF394) is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, characterized by tandem C2H2-type zinc finger motifs that facilitate sequence-specific DNA or RNA binding. Encoded by the *ZNF394* gene on human chromosome 8q24.3. this protein is believed to function as a transcriptional regulator, modulating gene expression by interacting with chromatin or recruiting epigenetic modifiers. While its precise biological role remains under investigation, ZNF394 has been implicated in cellular processes such as proliferation, differentiation, and stress response. Emerging studies suggest its potential involvement in carcinogenesis, with altered expression observed in certain cancers, though mechanistic details are limited. Recombinant human ZNF394 protein, typically produced in *E. coli* or mammalian expression systems, is utilized to study DNA-protein interactions, signaling pathways, and its regulatory targets. Its structural domains, including the KRAB repression module and zinc finger array, make it valuable for in vitro assays exploring transcriptional control mechanisms. Current research focuses on elucidating its physiological ligands, downstream genes, and therapeutic relevance in diseases linked to transcriptional dysregulation.
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