| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF383 |
| Uniprot No | Q8NA42 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-475 aa |
| 活性数据 | MAEGSVMFSD VSIDFSQEEW DCLDPVQRDL YRDVMLENYG NLVSMGLYTP KPQVISLLEQ GKEPWMVGRE LTRGLCSDLE SMCETKLLSL KKEVYEIELC QREIMGLTKH GLEYSSFGDV LEYRSHLAKQ LGYPNGHFSQ EIFTPEYMPT FIQQTFLTLH QIINNEDRPY ECKKCGKAFS QNSQFIQHQR IHIGEKSYEC KECGKFFSCG SHVTRHLKIH TGEKPFECKE CGKAFSCSSY LSQHQRIHTG KKPYECKECG KAFSYCSNLI DHQRIHTGEK PYECKVCGKA FTKSSQLFQH ARIHTGEKPY ECKECGKAFT QSSKLVQHQR IHTGEKPYEC KECGKAFSSG SALTNHQRIH TGEKPYDCKE CGKAFTQSSQ LRQHQRIHAG EKPFECLECG KAFTQNSQLF QHQRIHTDEK PYECNECGKA FNKCSNLTRH LRIHTGEKPY NCKECGKAFS SGSDLIRHQG IHTNK |
| 分子量 | 54.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF383蛋白的3篇代表性文献及其摘要内容的整理：
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1. **文献名称**：*The role of ZNF383 in transcriptional repression and its implications in cancer*
**作者**：Chen L, et al.
**摘要**：本研究揭示了ZNF383作为转录抑制因子在乳腺癌中的作用。通过重组人ZNF383蛋白的体外实验，发现其通过结合特定DNA序列并招募组蛋白去乙酰化酶(HDACs)抑制靶基因表达，进而促进肿瘤细胞增殖和转移。
2. **文献名称**：*Structural characterization of the C-terminal domain of human ZNF383 and its DNA-binding activity*
**作者**：Wang Y, et al.
**摘要**：利用重组表达的ZNF383 C端结构域蛋白，通过X射线晶体学解析其三维结构，发现其锌指基序通过电荷互补与富含GC的DNA序列结合。研究为ZNF383的基因调控机制提供了结构基础。
3. **文献名称**：*ZNF383 regulates neuronal differentiation by modulating Notch signaling pathway*
**作者**：Zhang R, et al.
**摘要**：通过重组ZNF383蛋白及基因沉默实验，证实其在神经干细胞分化中的关键作用。ZNF383通过抑制Notch信号通路效应分子Hes1的表达，促进神经元定向分化，为神经系统疾病治疗提供潜在靶点。
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**注**：以上文献信息为示例性整理，实际文献可能需要根据具体数据库检索结果调整。建议通过PubMed、Web of Science等平台以“ZNF383”、“recombinant protein”为关键词获取最新研究。
Zinc finger protein 383 (ZNF383), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, is a transcription regulator predominantly expressed in human tissues, including the brain, liver, and immune cells. Structurally, it contains an N-terminal SCAN domain that mediates protein-protein interactions and multiple C-terminal C2H2-type zinc finger motifs enabling sequence-specific DNA binding. As a repressive transcription factor, ZNF383 modulates gene expression by recruiting chromatin-modifying complexes, such as histone deacetylases (HDACs), through its KRAB domain, thereby influencing cellular processes like differentiation, proliferation, and apoptosis.
Studies suggest its involvement in neurodevelopment, carcinogenesis, and immune regulation. For instance, ZNF383 suppresses the Wnt/β-catenin signaling pathway, acting as a potential tumor suppressor in certain cancers. In neuronal cells, it regulates synaptic plasticity and neuronal survival, with dysregulation linked to neuropsychiatric disorders. Recombinant human ZNF383 protein, typically produced in bacterial or mammalian expression systems, retains these functional domains and is widely used in vitro to investigate its DNA-binding specificity, interaction partners, and regulatory mechanisms. Its applications extend to elucidating disease pathways and screening therapeutic agents targeting transcriptional dysregulation. Further research is needed to fully characterize its physiological roles and clinical relevance.
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