| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF366 |
| Uniprot No | Q8N895 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-744 aa |
| 活性数据 | MQKEMKMIKD EDVHFDLAVK KTPSFPHCLQ PVASRGKAPQ RHPFPEALRG PFSQFRYEPP PGDLDGFPGV FEGAGSRKRK SMPTKMPYNH PAEEVTLALH SEENKNHGLP NLPLLFPQPP RPKYDSQMID LCNVGFQFYR SLEHFGGKPV KQEPIKPSAV WPQPTPTPFL PTPYPYYPKV HPGLMFPFFV PSSSPFPFSR HTFLPKQPPE PLLPRKAEPQ ESEETKQKVE RVDVNVQIDD SYYVDVGGSQ KRWQCPTCEK SYTSKYNLVT HILGHSGIKP HACTHCGKLF KQLSHLHTHM LTHQGTRPHK CQVCHKAFTQ TSHLKRHMMQ HSEVKPHNCR VCGRGFAYPS ELKAHEAKHA SGRENICVEC GLDFPTLAQL KRHLTTHRGP IQYNCSECDK TFQYPSQLQN HMMKHKDIRP YICSECGMEF VQPHHLKQHS LTHKGVKEHK CGICGREFTL LANMKRHVLI HTNIRAYQCH LCYKSFVQKQ TLKAHMIVHS DVKPFKCKLC GKEFNRMHNL MGHMHLHSDS KPFKCLYCPS KFTLKGNLTR HMKVKHGVME RGLHSQGLGR GRIALAQTAG VLRSLEQEEP FDLSQKRRAK VPVFQSDGES AQGSHCHEEE EEDNCYEVEP YSPGLAPQSQ QLCTPEDLST KSEHAPEVLE EACKEEKEDA SKGEWEKRSK GDLGAEGGQE RDCAGRDECL SLRAFQSTRR GPSFSDYLYF KHRDESLKEL LERKMEKQAV LLGI |
| 分子量 | 85.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇关于重组人ZNF366蛋白的模拟参考文献示例(实际文献可能需要通过学术数据库查询获取)：
---
1. **文献名称**：*ZNF366 is an estrogen receptor-associated transcriptional repressor and modulates hormone-dependent breast cancer*
**作者**：Smith A, et al.
**摘要**：研究证明ZNF366与雌激素受体(ER)相互作用，抑制雌激素依赖性基因转录，并参与乳腺癌细胞的增殖调控，提示其在激素依赖性癌症中的潜在作用。
2. **文献名称**：*Recombinant ZNF366 protein inhibits C/EBPβ-mediated transcriptional activation in adipogenesis*
**作者**：Chen L, et al.
**摘要**：通过体外表达重组ZNF366蛋白，发现其通过与转录因子C/EBPβ结合，抑制脂肪生成相关基因的表达，表明ZNF366在代谢调控中的功能。
3. **文献名称**：*Structural and functional analysis of the recombinant ZNF366 protein in epigenetic silencing*
**作者**：Wang Y, et al.
**摘要**：解析重组ZNF366蛋白的结构，发现其锌指结构域能够招募HDAC复合物，介导组蛋白去乙酰化，进而参与表观遗传沉默过程。
4. **文献名称**：*ZNF366 knockdown enhances cisplatin sensitivity in ovarian cancer via DNA damage response*
**作者**：Kim H, et al.
**摘要**：研究利用重组ZNF366蛋白进行功能验证，发现其通过调控DNA损伤修复通路影响卵巢癌细胞对化疗药物的敏感性。
---
**注**：以上文献为模拟示例，如需实际文献，建议通过PubMed、Web of Science或Google Scholar检索关键词“ZNF366 recombinant”或“ZNF366 function”获取。
Zinc finger protein 366 (ZNF366), also known as epithelial stromal interaction 1 (EPSTI1), is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, characterized by multiple C2H2-type zinc finger motifs that mediate nucleic acid binding. Initially identified as a transcriptional co-regulator, ZNF366 plays critical roles in transcriptional repression, epigenetic regulation, and cellular signaling pathways. It interacts with nuclear receptors, including glucocorticoid receptors (GR) and estrogen receptors (ER), modulating their transcriptional activity. Studies highlight its involvement in embryonic development, cell differentiation, and cancer progression, particularly in breast and ovarian cancers. For example, ZNF366 is implicated in regulating hypoxia-inducible factor 1α (HIF-1α)-mediated pathways, influencing tumor angiogenesis and metastasis.
Recombinant human ZNF366 protein is produced using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His or GST) for purification and detection. Its structural domains, including the KRAB and zinc finger regions, enable DNA binding and protein-protein interactions. Researchers employ recombinant ZNF366 to study its molecular mechanisms, such as chromatin remodeling via histone deacetylase (HDAC) recruitment, or its role in DNA damage response. Dysregulation of ZNF366 is associated with chemoresistance in cancers, making it a potential therapeutic target. Current investigations focus on its dual oncogenic or tumor-suppressive roles, likely context-dependent, and its utility in developing targeted therapies or biomarkers.
×
