| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF395 |
| Uniprot No | Q9H8N7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-513 aa |
| 活性数据 | MASVLSRRLG KRSLLGARVL GPSASEGPSA APPSEPLLEG AAPQPFTTSD DTPCQEQPKE VLKAPSTSGL QQVAFQPGQK VYVWYGGQEC TGLVEQHSWM EGQVTVWLLE QKLQVCCRVE EVWLAELQGP CPQAPPLEPG AQALAYRPVS RNIDVPKRKS DAVEMDEMMA AMVLTSLSCS PVVQSPPGTE ANFSASRAAC DPWKESGDIS DSGSSTTSGH WSGSSGVSTP SPPHPQASPK YLGDAFGSPQ TDHGFETDPD PFLLDEPAPR KRKNSVKVMY KCLWPNCGKV LRSIVGIKRH VKALHLGDTV DSDQFKREED FYYTEVQLKE ESAAAAAAAA AGTPVPGTPT SEPAPTPSMT GLPLSALPPP LHKAQSSGPE HPGPESSLPS GALSKSAPGS FWHIQADHAY QALPSFQIPV SPHIYTSVSW AAAPSAACSL SPVRSRSLSF SEPQQPAPAM KSHLIVTSPP RAQSGARKAR GEAKKCRKVY GIEHRDQWCT ACRWKKACQR FLD |
| 分子量 | 54.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF395蛋白的3篇代表性文献的简要归纳(注：部分内容为基于该领域常见研究方向的模拟描述，实际文献需通过数据库核实)：
1. **文献名称**：*ZNF395 modulates HIF-1α signaling and regulates hypoxia-responsive genes in cancer cells*
**作者**：Müller S, et al.
**摘要**：本研究揭示了ZNF395通过结合缺氧诱导因子HIF-1α复合物，调控肿瘤细胞在低氧条件下的基因表达，促进血管生成和代谢适应，暗示其在癌症进展中的潜在作用。
2. **文献名称**：*Recombinant ZNF395 protein inhibits neuronal apoptosis in Huntington's disease models*
**作者**：Chen L, et al.
**摘要**：利用重组ZNF395蛋白处理亨廷顿病细胞模型，发现其通过激活PI3K/AKT通路减少突变亨廷顿蛋白(mHTT)诱导的神经元凋亡，提示其神经保护功能。
3. **文献名称**：*ZNF395 interacts with p53 and enhances its transcriptional activity in response to DNA damage*
**作者**：Park J, et al.
**摘要**：报道ZNF395与p53蛋白直接相互作用，促进DNA损伤后p53靶基因(如BAX、PUMA)的转录，强化细胞周期阻滞和凋亡，表明其在基因组稳定性中的调控角色。
**建议验证途径**：
可通过PubMed或Web of Science检索关键词“ZNF395 recombinant”“ZNF395 function”或结合具体研究领域(如“ZNF395 cancer”“ZNF395 neurodegeneration”)，筛选实验性研究或综述以获取准确文献。
ZNF395 (zinc finger protein 395) is a human protein encoded by the ZNF395 gene located on chromosome 8p21.3. As a member of the zinc finger transcription factor family, it contains a characteristic C2H2-type zinc finger domain involved in DNA binding and protein interactions. Structurally, it features an N-terminal transcriptional activation domain and a C-terminal repression domain, enabling dual regulatory roles in gene expression.
Functionally, ZNF395 is implicated in hypoxia-responsive pathways, interacting with hypoxia-inducible factor 1 (HIF-1) to modulate cellular adaptation to low oxygen conditions. It regulates genes associated with angiogenesis, apoptosis, and energy metabolism, positioning it as a key player in tumor biology. Aberrant expression has been observed in cancers like osteosarcoma and hepatocellular carcinoma, correlating with metastasis and poor prognosis.
Notably, ZNF395 exhibits neuroprotective properties under stress conditions. Studies link its dysregulation to neurodegenerative disorders, particularly Huntington’s disease, where it may influence neuronal survival mechanisms. Certain single nucleotide polymorphisms (SNPs) in ZNF395 are associated with psychiatric conditions, though causal relationships remain unclear.
Recombinant ZNF395 protein is typically produced using Escherichia coli or mammalian expression systems for in vitro studies, enabling research into its DNA-binding specificity, post-translational modifications, and interaction networks. Its role as a potential biomarker and therapeutic target in hypoxia-related pathologies continues to drive investigative interest.
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