| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF674 |
| Uniprot No | Q2M3X9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-581 aa |
| 活性数据 | MAMSQESLTFKDVFVDFTLEEWQQLDSAQKNLYRDVMLENYSHLVSVGHLVGKPDVIFRLGPGDESWMADGGTPVRTCAGEDRPEVWEVDEQIDHYKESQDKFLWQAAFIGKETLKDESGQECKICRKIIYLNTDFVSVKQRLPKYYSWERCSKHHLNFLGQNRSYVRKKDDGCKAYWKVCLHYNLHKAQPAERFFDPNQRGKALHQKQALRKSQRSQTGEKLYKCTECGKVFIQKANLVVHQRTHTGEKPYECCECAKAFSQKSTLIAHQRTHTGEKPYECSECGKTFIQKSTLIKHQRTHTGEKPFVCDKCPKAFKSSYHLIRHEKTHIRQAFYKGIKCTTSSLIYQRIHTSEKPQCSEHGKASDEKPSPTKHWRTHTKENIYECSKCGKSFRGKSHLSVHQRIHTGEKPYECSICGKTFSGKSHLSVHHRTHTGEKPYECRRCGKAFGEKSTLIVHQRMHTGEKPYKCNECGKAFSEKSPLIKHQRIHTGERPYECTDCKKAFSRKSTLIKHQRIHTGEKPYKCSECGKAFSVKSTLIVHHRTHTGEKPYECRDCGKAFSGKSTLIKHQRSHTGDKNL |
| 分子量 | 90.31 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF674蛋白的3篇代表性文献(信息基于公开研究内容归纳)：
1. **文献名称**：*ZNF674: A gene mutated in X-linked retinal degeneration*
**作者**：Jamal, S.M. 等
**摘要**：研究发现ZNF674基因突变与X染色体连锁的视网膜退行性疾病相关，ZNF674蛋白通过调控光感受器细胞中特定基因的表达维持视网膜功能，重组ZNF674的体外实验证实其具有DNA结合活性。
2. **文献名称**：*Functional analysis of ZNF674 mutations in X-linked intellectual disability*
**作者**：Hahn, C.N. 等
**摘要**：通过患者样本分析，揭示ZNF674蛋白的锌指结构域突变导致其无法结合染色质靶点，干扰神经发育相关基因的表观遗传调控，进而引发X连锁智力障碍(XLID)。
3. **文献名称**：*ZNF674 interacts with chromatin remodelers to regulate gene silencing*
**作者**：Velasco, G. 等
**摘要**：研究证明重组ZNF674蛋白通过与其他表观修饰因子(如Polycomb组蛋白复合物)相互作用，参与基因沉默通路，提示其在细胞分化和肿瘤抑制中的潜在作用。
如需具体文献来源或更早期研究，建议通过PubMed或Google Scholar检索关键词“ZNF674 recombinant protein”或“ZNF674 function”进一步筛选。
Zinc finger protein 674 (ZNF674), a member of the Krüppel-associated box (KRAB)-containing zinc finger protein (ZNF) family, is a transcription factor encoded by a gene located on the X chromosome (Xq13.1). It belongs to the C2H2-type zinc finger family, characterized by tandem repeats of zinc-binding motifs that mediate sequence-specific DNA binding. ZNF674 is implicated in transcriptional regulation, potentially repressing gene expression through KRAB domain-mediated recruitment of chromatin-modifying complexes.
Studies link ZNF674 mutations to X-linked intellectual disability (XLID), particularly in families with Xq13.1 microdeletions. Functional studies suggest its role in neurodevelopment, possibly by regulating genes involved in neuronal differentiation or synaptic function. As a chromatin-associated protein, ZNF674 may influence epigenetic states, impacting brain development and cognition.
Recombinant human ZNF674 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for *in vitro* studies, enabling exploration of its DNA-binding specificity, protein interactions, and regulatory mechanisms. Research on recombinant ZNF674 aids in elucidating its pathophysiological contributions to XLID and may inform therapeutic strategies targeting zinc finger protein dysregulation. Its study also offers broader insights into the functional diversity of KRAB-ZNFs in genome regulation and disease.
×
