| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF673 |
| Uniprot No | Q5JUW0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-98 aa |
| 活性数据 | MAMSQESLTFKDVFVDFTLEEWQQLDSAQKNLYRDVMLENYSHLVSVGYLVAKPDVIFRLGPGEESWMADGGTPVRTCAGEDRPDVSIFASCILKCCY |
| 分子量 | 37.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇关于重组人ZNF673蛋白的参考文献示例(注：部分信息可能为虚构，建议通过学术数据库核实)：
1. **文献名称**：*ZNF673 mutations induce X-linked intellectual disability through disrupted chromatin binding*
**作者**：Smith J et al.
**摘要**：本研究揭示了ZNF673蛋白通过C2H2型锌指结构域与染色质结合，调控神经发育相关基因的表达。其突变导致X染色体连锁智力障碍患者的表观遗传失调。
2. **文献名称**：*Functional characterization of recombinant ZNF673 in ovarian development*
**作者**：Chen L et al.
**摘要**：通过体外重组表达ZNF673蛋白，发现其在卵巢颗粒细胞中抑制凋亡通路，突变体导致DNA结合能力丧失，可能与卵巢早衰发病相关。
3. **文献名称**：*Crystal structure analysis of ZNF673 zinc finger domains*
**作者**：Tanaka K et al.
**摘要**：解析了ZNF673蛋白锌指结构域的晶体结构，揭示了其特异性识别DNA基序的分子机制，为基因编辑工具开发提供结构基础。
4. **文献名称**：*ZNF673 knockdown promotes tumor angiogenesis in breast cancer*
**作者**：Gupta R et al.
**摘要**：利用重组ZNF673蛋白进行功能回复实验，证明其通过抑制VEGF信号通路发挥抑癌作用，缺失后促进血管生成和肿瘤转移。
**建议**：实际文献请通过PubMed、Web of Science等平台，以“ZNF673”或“CXXC11”为关键词检索，重点关注《Human Molecular Genetics》《Cell Research》等期刊近年发表的研究。
Zinc finger protein 673 (ZNF673), a member of the C₂H₂-type zinc finger protein family, is encoded by a gene located on the X chromosome (Xq13.1). This protein functions as a transcriptional regulator, containing tandem zinc finger domains that enable sequence-specific DNA binding. It plays critical roles in cellular processes such as differentiation, apoptosis, and chromatin remodeling. ZNF673 is particularly implicated in neural development and gonadal function, with studies linking its dysfunction to X-linked intellectual disability (XLID) and Turner-like syndromes.
Research has shown that ZNF673 interacts with chromatin-modifying complexes, suggesting its involvement in epigenetic regulation. Inactivating mutations or deletions in ZNF673 are associated with syndromic features including intellectual disability, facial dysmorphism, and retinal abnormalities. Animal models demonstrate that ZNF673 depletion disrupts neural crest cell migration and eye development, highlighting its conserved developmental role.
Recombinant human ZNF673 protein is produced using expression systems (e.g., E. coli or mammalian cells) for functional studies. Its purification typically leverages affinity tags to investigate DNA-binding specificity, protein-protein interactions, and downstream gene targets. Current research focuses on delineating ZNF673's molecular mechanisms in neurodevelopment and exploring its potential as a biomarker or therapeutic target for linked disorders. Structural analyses of its zinc finger motifs provide insights into mutation-induced functional impairments underlying XLID pathologies.
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