| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF658 |
| Uniprot No | Q5TYW1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-615 aa |
| 活性数据 | MSQASVSFQDVTVEFTREEWQHLGPVERTLYRDVMLENYSHLISVGYCITKPKVISKLEKGEEPWSLEDEFLNQRYPGYFKVDHIKGIREKQEKPLWQEIFISDADKTLSKEGQKVLEKPFNLEIAPELSEKISCKCDSHRMNLPVASQLIISERKYSRKKTEYMNVCEKLQLDIKHEKAHAEEKSYEHGENAKAFSYKKDQHWKFQTLEESFECDGSGQGLYDKTICITPQSFLTGEKSCKDDEFRKNFDKITLFNHMRTDTRGKCSDLNEYGTSCDKTTAVEYNKVHMAMTHYECNERGINFSRKSPLTQSQRTITGWSAFESNKCEENFSQSSAHIVHQKTQAGDKFGEHNECTDALYQKLDFTAHQRIHTEDKFYLSDEHGKCRKSFYRKAHLIQHQRPHSGEKTYQYEECAKSFCSSSHPIQHPGTYVGFKLYECNECGKAFCQNSNLSKHLRIHTKEKPCDNNGCGRSYKSPLIGHQKTDAEMELCGGSEYGKTSHLKGHQRILMGEKPYECIECGKTFSKTSHLRAHQRIHTGEKPYECVECEKTFSHKTHLSVHQRVHTGEKPYECNDCGKSFTYNSALRAHQRIHTGLAVCHVGILMATKSLFHQS |
| 分子量 | 97.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF658蛋白的参考文献示例(注：内容为模拟生成，实际文献需通过学术数据库验证)：
1. **文献名称**：*ZNF658 regulates metal homeostasis by binding to metal-responsive elements in the metallothionein promoters*
**作者**：Smith J, et al. (2018)
**摘要**：研究揭示了重组ZNF658蛋白通过锌指结构域特异性识别金属响应元件(MRE)，调控金属硫蛋白(MT)基因的表达，参与细胞内锌和铁等金属离子的稳态平衡。
2. **文献名称**：*Structural insights into the DNA-binding mechanism of the recombinant ZNF658 protein*
**作者**：Brown K, et al. (2020)
**摘要**：通过X射线晶体学解析重组ZNF658的锌指结构域与DNA复合物的三维结构，阐明其通过保守基序结合基因启动子区域的分子机制。
3. **文献名称**：*Recombinant ZNF658 suppresses oncogenic pathways in breast cancer cells*
**作者**：Zhang L, et al. (2021)
**摘要**：体外实验表明，重组ZNF658蛋白过表达可抑制乳腺癌细胞增殖，并通过下调促癌基因(如MYC)的转录活性发挥抑癌作用。
4. **文献名称**：*Optimization of recombinant ZNF658 expression in E. coli for functional studies*
**作者**：Patel R, et al. (2019)
**摘要**：报道了在大肠杆菌中高效表达可溶性重组ZNF658蛋白的优化策略，并验证其体外结合DNA的活性，为后续功能研究提供可靠工具。
**注意**：以上文献信息为示例，实际引用时请通过PubMed、Web of Science等平台核实具体作者、年份及摘要内容。
Zinc finger protein 658 (ZNF658) is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, characterized by multiple C2H2-type zinc finger motifs that mediate sequence-specific DNA binding. It functions as a transcriptional regulator, playing roles in cellular responses to environmental and metabolic cues. Initially identified through homology to zinc finger proteins, ZNF658 gained attention for its involvement in metal ion homeostasis. Studies show it binds to metal-responsive elements (MREs) in gene promoters, including those of SLC30A5 (ZnT5) and SLC30A10 (ZnT10), to regulate zinc and manganese transport. This activity links ZNF658 to cellular adaptation under metal stress and redox balance maintenance.
Recombinant human ZNF658 protein, typically produced in E. coli or mammalian expression systems, retains DNA-binding capacity and is used to study its molecular interactions and regulatory mechanisms. Structural analyses reveal conserved nuclear localization signals and KRAB domains, suggesting roles in chromatin remodeling and gene silencing through co-repressor recruitment. Emerging evidence implicates ZNF658 in broader biological contexts, including cancer progression (e.g., breast and liver cancers) and neurological disorders, potentially via dysregulation of metal-associated pathways. Its recombinant form enables biochemical assays, antibody production, and functional studies to explore its therapeutic potential or diagnostic value. Current research focuses on elucidating tissue-specific targets and post-translational modifications governing its activity.
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