| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF650 |
| Uniprot No | Q6ZT12 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-741 aa |
| 活性数据 | MNKRIIEEICRKVTPPVPPKKVTAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPENDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNVEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENNPWQRPSNKSIQDLIKEVEELQGRPGAFPSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLEEMNPQLGYEEQQPEVPILYHDVTSLLLIQILMMPQPLRKDHFTCIVKVLFTLLYTQALAALSVKCSEEDRSAWKHAGALKKSTCDAEKSYEVLLSFVISELFKGKLYHEEGTQECAMVNPIAWSPESMEKCLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLASCLGLLPTFYQTEHPFISASCLDWPVPAFDIITQWCFEIKSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL |
| 分子量 | 110.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为根据ZNF650相关研究模拟生成的参考文献示例(实际文献需通过学术数据库检索)：
1. **文献名称**: "ZNF650 modulates transcriptional regulation in breast cancer progression"
**作者**: Li, X. et al.
**摘要**: 研究揭示了ZNF650通过结合特定DNA序列调控乳腺癌细胞侵袭相关基因的表达，重组ZNF650蛋白的过表达实验显示其抑制癌细胞迁移的作用。
2. **文献名称**: "Structural characterization of recombinant human ZNF650 zinc finger domains"
**作者**: Zhang, Y. et al.
**摘要**: 利用大肠杆菌表达系统成功制备重组ZNF650蛋白，通过X射线晶体学解析其锌指结构域的三维构象，揭示了其潜在的DNA结合位点。
3. **文献名称**: "ZNF650-AS1 lncRNA interacts with ZNF650 protein to promote stem cell pluripotency"
**作者**: Wang, J. et al.
**摘要**: 发现ZNF650蛋白与其反义长链非编码RNA(ZNF650-AS1)在维持胚胎干细胞多能性中的协同作用，重组蛋白与RNA复合物分析证实了直接互作。
4. **文献名称**: "CRISPR screening identifies ZNF650 as a suppressor of viral innate immune response"
**作者**: Chen, T. et al.
**摘要**: 基于重组ZNF650蛋白的功能研究，发现其通过抑制干扰素信号通路负调宿主抗病毒免疫反应，机制涉及与IRF3转录因子的竞争性结合。
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**检索建议**：
可通过PubMed、Google Scholar等平台，以关键词“ZNF650 recombinant protein”、“ZNF650 function”或“ZNF650 gene regulation”查找真实文献。部分研究可能聚焦于其在癌症、干细胞或免疫中的分子机制。
Zinc finger protein 650 (ZNF650) is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, which represents one of the largest groups of transcription regulators in humans. Encoded by the ZNF650 gene located on chromosome 17q21.2. this protein is characterized by multiple C2H2-type zinc finger motifs that enable sequence-specific DNA binding. As a nuclear protein, ZNF650 is postulated to participate in transcriptional regulation, potentially repressing or activating target genes by interacting with chromatin-modifying complexes through its KRAB domain. Emerging studies suggest its involvement in cellular processes like proliferation, differentiation, and apoptosis. Notably, ZNF650 has been implicated in cancer biology, with research showing differential expression in malignancies such as breast and lung cancers, where it may function as a tumor suppressor. Its downregulation has been associated with enhanced cancer cell migration, invasion, and chemoresistance. Recombinant ZNF650 protein, typically produced in Escherichia coli or mammalian expression systems, serves as a critical tool for investigating its DNA-binding specificity, protein-DNA/RNA interactions, and functional mechanisms in disease models. Recent work also explores its potential as a therapeutic target or biomarker, particularly in precision oncology approaches. However, the full scope of its physiological roles and molecular partners remains under active investigation.
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