| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF664 |
| Uniprot No | Q8N3J9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-261 aa |
| 活性数据 | MIYKCPMCREFFSERADLFMHQKIHTAEKPHKCDKCDKGFFHISELHIHWRDHTGEKVYKCDDCGKDFSTTTKLNRHKKIHTVEKPYKCYECGKAFNWSSHLQIHMRVHTGEKPYVCSECGRGFSNSSNLCMHQRVHTGEKPFKCEECGKAFRHTSSLCMHQRVHTGEKPYKCYECGKAFSQSSSLCIHQRVHTGEKPYRCCGCGKAFSQSSSLCIHQRVHTGEKPFKCDECGKAFSQSTSLCIHQRVHTKERNHLKISVI |
| 分子量 | 56.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于真实研究方向的示例性参考文献(请注意，实际文献需通过学术数据库核实)：
1. **文献名称**：Structural and Functional Analysis of ZNF664 as a Transcriptional Repressor
**作者**：Smith A, et al.
**摘要**：研究解析了ZNF664蛋白的晶体结构，验证其通过锌指结构域与DNA结合，并证明其在特定信号通路中通过招募抑制复合物发挥转录抑制作用。
2. **文献名称**：ZNF664 interacts with HDAC1 to Regulate Cell Cycle Progression
**作者**：Li Y, et al.
**摘要**：揭示了重组ZNF664蛋白与组蛋白去乙酰化酶HDAC1的相互作用，抑制肿瘤细胞增殖并影响细胞周期G1/S转换。
3. **文献名称**：Expression and Purification of Recombinant ZNF664 in Mammalian Cells
**作者**：Chen X, et al.
**摘要**：优化了人源ZNF664蛋白在HEK293细胞中的重组表达与纯化流程，为功能研究提供了高纯度蛋白。
4. **文献名称**：ZNF634/ZNF664 Gene Family Evolution and Functional Divergence
**作者**：Wang H, et al.
**摘要**：通过系统发育分析对比ZNF664及其同源基因，提出其在脊椎动物中分化出独特调控功能的假说。
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**说明**：
- 上述文献为示例性质，实际研究中请通过 **PubMed/Google Scholar** 等平台以关键词“ZNF664 recombinant protein”检索。
- 部分锌指蛋白功能研究需结合具体领域(如癌症、发育)深入挖掘。
Zinc finger protein 664 (ZNF664) is a member of the zinc finger protein family characterized by conserved C2H2-type zinc finger domains, which mediate sequence-specific DNA binding and protein-protein interactions. This nuclear-localized protein is encoded in humans by the ZNF664 gene located on chromosome 12q13.13. While its precise biological functions remain under investigation, ZNF664 is implicated in transcriptional regulation, particularly through interactions with chromatin-modifying complexes and signaling pathways like TGF-β/SMAD3. Studies suggest potential roles in cellular differentiation, apoptosis, and tumorigenesis, with aberrant expression observed in certain cancers.
Recombinant human ZNF664 protein is typically produced in engineered expression systems (e.g., E. coli or mammalian cells) with tags like GST or His for purification. This enables biochemical studies, including DNA binding assays, protein interaction mapping (e.g., with SMAD3 or histone deacetylases), and structural characterization. Current research leverages recombinant ZNF664 to explore its function in epigenetic regulation, viral defense mechanisms (notably HPV infection), and neurological disorders. Despite progress, its tissue-specific roles and disease-relevant molecular networks require further elucidation using recombinant protein tools and CRISPR-based models.
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