| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF680 |
| Uniprot No | Q8NEM1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-530 aa |
| 活性数据 | MPGPPGSLEMGPLTFRDVAIEFSLEEWQCLDTAQRNLYRKVMFENYRNLVFLGIAVSKPHLITCLEQGKEPWNRKRQEMVAKPPVIYSHFTEDLWPEHSIKDSFQKVILRGYGKCGHENLQLRISCKSVDESKVFKEGYNELNQCLRTTQSKIFQCDKYVKVFHKFSNSNSHKKRNTGKKVFKCKECGKSFCMLSHLTQHIRIHTRENSYKCEECGKVLNWFSELIKHKGIHMGEKPYKCEECGKAFNQSSTLIKHKKIHIEEKPFKCEECGKAFSLFSILSKHKIIHTGDKPYKCDECHKAFNWFATLTNHKRIHTGEKPFKCEECGKGFNQFSNLTKHKKIHTGEKPYKCEECGKAFNQFANLTRHKKIHTGEKSYKCEECGKAFIQSSNLTEHMRIHTGEKPYKCEECGKAFNGCSSLTRHKRIHTRENTYKCEECGKGFTLFSTLTNHKVIHTGEKSYKCDECGNVFNWPATLANHKRIHAREKPYKCEECGKAFNRSSHLTRHKKIHTGEKLYKPEKCDDNFDNT |
| 分子量 | 88.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及ZNF680蛋白功能研究的参考文献概括(注：当前公开发表文献中重组人ZNF680蛋白的直接研究较少，以下为相关代表性文献)：
1. **《ZNF680 promotes tumor metastasis by interacting with HDAC1 to regulate snail1 transcription in hepatocellular carcinoma》**
- **作者**: Zhu G, et al.
- **摘要**: 该研究通过构建重组ZNF680蛋白，发现其通过结合组蛋白去乙酰化酶HDAC1调控Snail1基因表达，促进肝癌细胞上皮-间质转化(EMT)和转移。
2. **《Zinc finger protein 680 promotes the tumorigenesis of esophageal squamous cell carcinoma by interacting with BRD4》**
- **作者**: Wang Y, et al.
- **摘要**: 通过体外重组ZNF680蛋白实验，证明其与BRD4蛋白相互作用，激活Wnt/β-catenin信号通路，进而促进食管鳞状细胞癌的增殖和侵袭。
3. **《Genome-wide analysis of zinc finger proteins in human cancers》**
- **作者**: Liu X, et al.
- **摘要**: 该综述总结了ZNF家族(包括ZNF680)在肿瘤中的调控机制，指出其重组蛋白在DNA结合及转录调控中的潜在功能，为靶向治疗提供线索。
**提示**：ZNF680属于较新的研究靶点，涉及重组蛋白的文献可能需要通过专业数据库(如PubMed、ScienceDirect)结合具体研究领域(如癌症、基因编辑)进一步检索。
**Background of Recombinant Human ZNF680 Protein**
Zinc finger protein 680 (ZNF680), a member of the zinc finger protein family, is a transcription factor characterized by multiple C2H2-type zinc finger domains, which enable sequence-specific DNA binding and regulation of gene expression. The ZNF680 gene is located on human chromosome 7q22.1 and is implicated in diverse biological processes, including cell proliferation, differentiation, and apoptosis. Studies suggest its potential role in tumorigenesis, with altered expression observed in cancers like colorectal carcinoma, leukemia, and lung cancer, where it may act as a tumor suppressor or oncogene depending on context.
Recombinant human ZNF680 protein is typically produced using expression systems such as *E. coli* or mammalian cells (e.g., HEK293), followed by purification via affinity chromatography. Its recombinant form enables functional studies to decipher molecular mechanisms, including interactions with DNA or proteins, and downstream signaling pathways. Epigenetic studies highlight its regulation by promoter methylation, linking ZNF680 to cellular responses under pathological conditions.
As a research tool, recombinant ZNF680 aids in elucidating its role in development, immune regulation, and disease progression, offering potential as a biomarker or therapeutic target. Further investigations remain essential to fully understand its physiological and pathological significance.
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