| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF684 |
| Uniprot No | Q5T5D7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-378 aa |
| 活性数据 | MISFQESVTFQDVAVDFTAEEWQLLDCAERTLYWDVMLENYRNLISVGCPITKTKVILKVEQGQEPWMVEGANPHESSPESDYPLVDEPGKHRESKDNFLKSVLLTFNKILTMERIHHYNMSTSLNPMRKKSYKSFEKCLPPNLDLLKYNRSYTVENAYECSECGKAFKKKFHFIRHEKNHTRKKPFECNDCGKAYSRKAHLATHQKIHNGERPFVCNDCGKAFMHKAQLVVHQRLHTGEKPYECSQCGKTFTWNSSFNQHVKSHTLEKSFECKECGKTFRYSSSLYKHSRFHTGEKPYQCIICGKAFGNTSVLVTHQRIHTGEKPYSCIECGKAFIKKSHLLRHQITHTGEKPYECNRCGKAFSQKSNLIVHQKIHT |
| 分子量 | 70.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF684蛋白的3篇假设文献示例，仅供参考(具体文献需通过学术数据库验证)：
---
1. **文献名称**：*"Recombinant ZNF684 suppresses oncogenic pathways via transcriptional regulation in colorectal cancer"*
**作者**：Zhang, Y. et al. (2020)
**摘要**：研究通过在大肠癌细胞中表达重组人ZNF684蛋白，发现其能结合特定DNA序列并抑制Wnt/β-catenin信号通路，降低肿瘤细胞增殖和迁移能力。
---
2. **文献名称**：*"Structural insights into ZNF684-DNA interaction using recombinant protein crystallography"*
**作者**：Garcia, R. et al. (2022)
**摘要**：通过重组表达并纯化人ZNF684蛋白的锌指结构域，解析其与靶DNA结合的晶体结构，揭示关键残基在识别特定碱基序列中的作用机制。
---
3. **文献名称**：*"ZNF684 modulates neuronal differentiation via epigenetic regulation of stem cell genes"*
**作者**：Lee, J. et al. (2021)
**摘要**：利用重组ZNF684蛋白进行染色质免疫沉淀(ChIP-seq)，证明其通过招募组蛋白去乙酰化酶(HDACs)抑制多能性基因，促进神经干细胞向神经元分化。
---
**备注**：上述文献为模拟内容，实际研究中请通过PubMed、Web of Science等平台检索关键词“ZNF684 recombinant”“ZNF684 function”等获取准确信息。
Zinc finger protein 684 (ZNF684) is a member of the C2H2-type zinc finger protein family, characterized by conserved zinc-binding motifs that mediate sequence-specific DNA or RNA interactions. As a transcription factor, ZNF684 is postulated to regulate gene expression by binding to specific genomic regions, though its precise molecular targets and functional mechanisms remain under investigation. The protein is encoded by the *ZNF684* gene located on human chromosome 1. and its expression has been detected in various tissues, with higher levels observed in the brain, suggesting a potential role in neural development or function.
Recombinant human ZNF684 protein is artificially expressed in vitro, often using bacterial or mammalian expression systems, to enable biochemical and functional studies. Such studies aim to elucidate its DNA-binding specificity, interaction partners (e.g., chromatin-modifying enzymes), and involvement in cellular processes like differentiation or stress responses. Dysregulation of zinc finger proteins, including ZNF684. has been linked to diseases such as cancer and neurological disorders, though direct evidence connecting ZNF684 to pathology is limited.
Current research on recombinant ZNF684 focuses on mapping its binding sites genome-wide, characterizing structural features of its zinc finger domains, and exploring its regulatory roles in cellular models. This work may provide insights into its contribution to transcriptional networks and potential therapeutic applications, such as engineered zinc finger nucleases for genome editing. Despite progress, the full scope of ZNF684's biological significance remains an active area of exploration.
×
