| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF688 |
| Uniprot No | P0C7X2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-276 aa |
| 活性数据 | MAPPPAPLLAPRPGETRPGCRKPGTVSFADVAVYFSPEEWGCLRPAQRALYRDVMQETYGHLGALGFPGPKPALISWMEQESEAWSPAAQDPEKGERLGGARRGDVPNRKEEEPEEVPRAKGPRKAPVKESPEVLVERNPDPAISVAPARAQPPKNAAWDPTTGAQPPAPIPSMDAQAGQRRHVCTDCGRRFTYPSLLVSHRRMHSGERPFPCPECGMRFKRKFAVEAHQWIHRSCSGGRRGRRPGIRAVPRAPVRGDRDPPVLFRHYPDIFEECG |
| 分子量 | 57 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人ZNF688蛋白的示例参考文献(注：文献为虚构示例，建议通过学术数据库检索实际研究)：
1. **文献名称**：*ZNF688 interacts with BRCA1 to maintain genomic stability through homologous recombination repair*
**作者**：Zhang Y, et al.
**摘要**：研究发现ZNF688通过其锌指结构域与BRCA1蛋白结合，在DNA双链断裂修复中发挥关键作用，敲除ZNF688导致细胞对电离辐射敏感性增加，提示其在维持基因组稳定性中的功能。
2. **文献名称**：*Structural analysis of the zinc finger domains in human ZNF688 and its DNA-binding specificity*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析了ZNF688的C2H2型锌指结构域，发现其特异识别富含GC的DNA序列，为理解其在转录调控中的作用提供结构基础。
3. **文献名称**：*Overexpression of ZNF688 promotes hepatocellular carcinoma progression via Wnt/β-catenin signaling*
**作者**：Wang X, et al.
**摘要**：在肝细胞癌中，ZNF688通过激活Wnt通路促进肿瘤细胞增殖和转移，临床样本分析显示其高表达与患者预后不良显著相关。
4. **文献名称**：*Recombinant expression and purification of ZNF688 protein for functional screening*
**作者**：Kim H, et al.
**摘要**：建立了一种在大肠杆菌中高效表达并纯化重组人ZNF688蛋白的方法，验证其与靶DNA结合活性，为后续高通量药物筛选奠定基础。
建议通过PubMed、Web of Science或Google Scholar检索实际文献，关键词：*ZNF688 recombinant protein, zinc finger protein 688 function*。
Zinc finger protein 688 (ZNF688), a member of the zinc finger protein family, is characterized by its evolutionarily conserved C2H2-type zinc finger domains, which facilitate sequence-specific DNA binding. This human protein, encoded by the *ZNF688* gene on chromosome 16q24.3. is postulated to function as a transcription regulator, potentially influencing gene expression by interacting with promoter regions or chromatin-modifying complexes. Though less studied compared to other zinc finger proteins, emerging evidence links ZNF688 to cellular processes such as proliferation, differentiation, and apoptosis. Its dysregulation has been implicated in certain cancers, including leukemia and solid tumors, where altered expression may contribute to oncogenic or tumor-suppressive roles depending on cellular context.
Recombinant human ZNF688 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable biochemical and functional studies. Purified recombinant ZNF688 allows researchers to analyze its DNA-binding specificity, protein-protein interactions, and structural properties. Studies leveraging this protein have explored its potential involvement in signaling pathways, such as TP53 or Wnt, though mechanistic details remain under investigation. Recent work also highlights its possible role in stem cell biology and viral defense mechanisms. While further validation is needed, recombinant ZNF688 serves as a critical tool for deciphering its molecular functions and therapeutic relevance in disease models.
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