| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF699 |
| Uniprot No | Q32M78 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-642 aa |
| 活性数据 | MEEERKTAELQKNRIQDSVVFEDVAVDFTQEEWALLDLAQRNLYRDVMLENFQNLASLGYPLHTPHLISQWEQEEDLQTVKRELIQGIFMGEHREGFETQLKTNESVASQDICGEKISNEQKIVRFKRNDSWFSSLHENQESCGIDYQNKSHERHLRNHMVENIYECYEENQDGQTFSQVPNLDSLKRNTEVKSCECHECGKAFVDHSSLKSHIRSHTGSKPYQCKECGKAFHFLACFKKHMKTPTEEKPYECKECTKAFSCSSFFRAHMKIHIGKTNYECKECGKGFSCSSSLTEHKRIHSGDKPYECKECGKAFSCSSSLSKHKRIHSGDKPYECKECGKAFSSSSHLIIHIRIHTGEKPYECKECGKAFSESSKLTVHGRTHTGEKPYKCKECGKAYNCPSSLSIHMRKHTGEKPYECLECGKAFYLPTSLNTHVKNQSREKPYECKECGKAFSCPSSFRAHVRDHTGKIQYECKECGKTFSRSSSLTEHLRTHSGEKPYECKECGKAFISSSHLTVHIRTHTGEKPYECKKCGKAFIYPSALRIHMRTHTGEKPYECKECGKAFRHSSYLTVHARMHTGEKPFECLECGKAFSCPSSFRRHVRSHTGEKPYECKECGKAFVCPAYFRRHVKTHTRENI |
| 分子量 | 70.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人ZNF699蛋白的研究目前公开文献较少，以下为根据领域常见研究方向构建的**假设性参考文献示例**(非真实存在)，供参考思路：
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1. **标题**：*Cloning and Expression of Recombinant Human ZNF699 in Prokaryotic Systems*
**作者**：Lee, S. et al.
**摘要**：研究报道了通过原核表达系统(大肠杆菌)成功克隆并纯化重组人ZNF699蛋白，优化了诱导条件以提高可溶性蛋白产量，并通过质谱验证其正确性，为后续功能研究奠定基础。
2. **标题**：*ZNF699 Acts as a Transcriptional Repressor in Neural Stem Cell Differentiation*
**作者**：Zhang, Y. et al.
**摘要**：利用重组ZNF699蛋白进行体外实验，发现其通过结合特定DNA序列抑制下游基因转录，调控神经干细胞的增殖与分化，提示其在神经发育中的潜在作用。
3. **标题**：*Association of ZNF699 Gene Polymorphisms with Autoimmune Diseases*
**作者**：Gupta, R. et al.
**摘要**：通过病例对照研究，发现ZNF699基因多态性与类风湿关节炎易感性相关，并推测其编码的蛋白可能通过免疫调控通路参与疾病发生，需进一步功能验证。
4. **标题**：*Identification of ZNF699-Interacting Proteins by Yeast Two-Hybrid Screening*
**作者**：Wang, H. et al.
**摘要**：利用酵母双杂交技术筛选出ZNF699的多个互作蛋白(如组蛋白修饰酶和染色质重塑因子)，提示其可能通过表观遗传调控参与细胞周期进程。
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**实际检索建议**：
- 使用 **PubMed** 或 **Google Scholar** 查询“ZNF699”或“Zinc Finger Protein 699”，结合关键词“recombinant”(重组)、“function”(功能)等。
- 若文献有限，可扩展至锌指蛋白家族(如其他ZNF成员)的研究，或关注其所在基因组区域关联的疾病(如癌症、神经系统疾病)。
- 建议通过 **UniProt** 数据库(ID：需核实)获取ZNF699的基础生物学信息，如结构域和潜在功能线索。
ZNF699 (Zinc Finger Protein 699) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (ZFP) family, characterized by tandem C2H2-type zinc finger motifs that facilitate sequence-specific DNA binding. These proteins are involved in transcriptional regulation, chromatin remodeling, and epigenetic silencing. ZNF699 is encoded by the human genome and is thought to play roles in cellular differentiation, development, and disease pathways. Its KRAB domain typically recruits co-repressors like KAP1 to establish heterochromatin and suppress gene expression, while its zinc finger array enables targeting to specific genomic loci.
Recombinant human ZNF699 protein is engineered for in vitro studies to elucidate its molecular functions, DNA-binding specificity, and interactions with partner proteins. Produced using expression systems like *E. coli* or mammalian cells, it is often tagged (e.g., His, GST) for purification and detection. Research on ZNF699 may shed light on its potential involvement in cancer, neurological disorders, or viral defense mechanisms, as KRAB-ZFPs are implicated in these processes. However, its exact physiological roles remain less characterized compared to other ZFPs. Current applications include structural studies, promoter binding assays, and screening for small-molecule modulators. Further investigations are needed to define its regulatory networks and therapeutic relevance.
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