| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | AATK |
| Uniprot No | Q80YE4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 8-411aa |
| 氨基酸序列 | PSFAFSSHFDPDGAPLSELSWSSSLAVVAVSFSGIFTVVILMLACLCCKKGGIGFKEFENAEGDEYVADFSEQGSPAAAAQTGPDVYVLPLTEVSLPMAKQPGRSVQLLKSTDLGRHSLLYLKEIGHGWFGKVFLGEVHSGVSGTQVVVKELKVSASVQEQMQFLEEAQPYRALQHSNLLQCLAQCAEVTPYLLVMEFCPLGDLKGYLRSCRVTESMAPDPLTLQRMACEVACGVLHLHRHNYVHSDLALRNCLLTADLTVKVGDYGLSHCKYREDYLVTADQLWVPLRWIAPELVDEVHGNLLVVDQTKSSNVWSLGVTIWELFELGAQPYPQHSDRQVLAYAVREQQLKLPKPQLQLALSDRWYEVMQFCWLQPEQRPTAEEVHLLLSYLCAKGTTELEEEF |
| 预测分子量 | 71 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AATK(Apoptosis-Associated Tyrosine Kinase)重组蛋白的示例参考文献(模拟内容，仅供参考，建议通过学术数据库获取真实文献)：
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1. **文献名称**：*Functional Characterization of Recombinant AATK in Neuronal Apoptosis*
**作者**：Smith J, et al.
**摘要**：研究利用大肠杆菌表达系统成功纯化重组AATK蛋白，发现其激酶活性可诱导神经元细胞凋亡，并通过磷酸化下游靶点调控线粒体途径。
2. **文献名称**：*Expression and Purification of AATK in Mammalian Cells for Structural Studies*
**作者**：Lee H, Wang Y.
**摘要**：报道了哺乳动物细胞(HEK293)中重组AATK蛋白的高效表达与纯化方法，结合X射线晶体学解析其激酶结构域的三维构象，揭示其底物结合特性。
3. **文献名称**：*AATK Recombinant Protein as a Therapeutic Target in Cancer*
**作者**：Garcia R, et al.
**摘要**：通过体外实验证明重组AATK蛋白在肿瘤细胞中激活促凋亡通路，并筛选小分子抑制剂阻断其活性，为癌症治疗提供潜在策略。
4. **文献名称**：*Role of AATK in Autophagy: Insights from Recombinant Protein Kinase Assays*
**作者**：Chen L, et al.
**摘要**：利用重组AATK蛋白进行体外激酶反应实验，发现其通过磷酸化自噬相关蛋白(如Beclin-1)调控细胞自噬与凋亡的交叉对话。
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**注意**：以上内容为模拟示例，实际文献需通过PubMed、Web of Science或Google Scholar等平台检索关键词(如“AATK recombinant protein”或“Apoptosis-Associated Tyrosine Kinase expression”)获取。
The AATK (Apoptosis-Associated Tyrosine Kinase) recombinant protein is derived from the AATK gene, which encodes a serine/threonine kinase initially identified for its role in programmed cell death. Discovered in the mid-1990s, AATK is predominantly expressed in neuronal tissues and hematopoietic cells, suggesting dual functions in neural development and apoptosis regulation. Structurally, AATK contains an N-terminal kinase domain and a C-terminal proline-rich region involved in protein-protein interactions, enabling its participation in signaling cascades.
Research highlights AATK's involvement in neuronal differentiation, where it regulates cytoskeletal dynamics and axonal outgrowth by phosphorylating substrates like microtubule-associated proteins. Paradoxically, AATK also induces apoptosis in cancer cells under stress conditions, positioning it as a potential tumor suppressor. Dysregulation of AATK has been linked to neurodegenerative disorders and malignancies, driving interest in its molecular mechanisms.
Recombinant AATK proteins are typically produced in mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications. These proteins serve as critical tools for in vitro kinase assays, substrate identification, and drug discovery targeting kinase activity. Recent studies explore AATK's interplay with autophagy pathways and its role in chemoresistance, expanding its therapeutic relevance. Despite progress, AATK's full interactome and context-dependent functions (pro-survival vs. pro-apoptotic) remain under investigation, necessitating further structural and functional studies to unlock its diagnostic or therapeutic potential.
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