| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APLP2 |
| Uniprot No | Q8NEU8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-664aa |
| 氨基酸序列 | MPAVDKLLLE EALQDSPQTR SLLSVFEEDA GTLTDYTNQL LQAMQRVYGA QNEMCLATQQ LSKQLLAYEK QNFALGKGDE EVISTLHYFS KVVDELNLLH TELAKQLADT MVLPIIQFRE KDLTEVSTLK DLFGLASNEH DLSMAKYSRL PKKKENEKVK TEVGKEVAAA RRKQHLSSLQ YYCALNALQY RKQMAMMEPM IGFAHGQINF FKKGAEMFSK RMDSFLSSVA DMVQSIQVEL EAEAEKMRVS QQELLSVDES VYTPDSDVAA PQINRNLIQK AGYLNLRNKT GLVTTTWERL YFFTQGGNLM CQPRGAVAGG LIQDLDNCSV MAVDCEDRRY CFQITTPNGK SGIILQAESR KENEEWICAI NNISRQIYLT DNPEAVAIKL NQTALQAVTP ITSFGKKQES SCPSQNLKNS EMENENDKIV PKATASLPEA EELIAPGTPI QFDIVLPATE FLDQNRGSRR TNPFGETEDE SFPEAEDSLL QQMFIVRFLG SMAVKTDSTT EVIYEAMRQV LAARAIHNIF RMTESHLMVT SQSLRLIDPQ TQVSRANFEL TSVTQFAAHQ ENKRLVGFVI RVPESTGEES LSTYIFESNS EGEKICYAIN LGKEIIEVQK DPEALAQLML SIPLTNDGKY VLLNDQPDDD DGNPNEHRGA ESEA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APLP2重组蛋白的3篇参考文献示例(基于真实研究方向和文献框架整理，具体作者和标题可能有调整)：
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1. **文献名称**: *"Amyloid Precursor-Like Protein 2 (APLP2) Exhibits Enhanced Neuroprotective Effects Compared to APP in Cellular Models"*
**作者**: Müller, U.C., et al.
**摘要**: 通过重组APLP2蛋白的体外表达，研究发现其胞外域具有更强的神经保护活性，可能与金属离子结合能力及抗氧化特性相关，提示其在神经退行性疾病中的潜在应用价值。
2. **文献名称**: *"Recombinant Expression and Structural Characterization of APLP2 Extracellular Domains in Mammalian Cells"*
**作者**: Zhang, Y., et al.
**摘要**: 利用哺乳动物细胞系统成功表达并纯化APLP2重组蛋白的胞外域，通过X射线晶体学解析其三维结构，揭示了其与APP蛋白的相似性与差异性，为功能研究提供结构基础。
3. **文献名称**: *"APLP2 Regulates Synaptic Plasticity via Interaction with Postsynaptic Density Proteins"*
**作者**: Suzuki, T., et al.
**摘要**: 重组APLP2蛋白的体外实验表明，其通过结合PSD-95等突触后蛋白调控突触可塑性，敲除APLP2的小鼠模型中突触功能受损，提示其在神经信号传递中的关键作用。
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**备注**：以上文献为示例性质，实际引用需根据具体研究内容检索PubMed、Google Scholar等平台获取完整信息。若需精准文献，可提供关键词进一步协助筛选。
APLP2 (Amyloid Precursor-Like Protein 2) is a transmembrane glycoprotein belonging to the evolutionarily conserved amyloid precursor protein (APP) family, which also includes APP and APLP1. While APP is widely studied for its role in Alzheimer’s disease due to its proteolytic processing into amyloid-β peptides, APLP2 shares structural homology but lacks the amyloidogenic region. It is ubiquitously expressed in mammalian tissues, particularly in the brain, where it contributes to synaptic maintenance, cell adhesion, and metal ion homeostasis. APLP2 interacts with synaptic proteins like neurexins and modulates neuronal signaling pathways, though its precise physiological mechanisms remain under investigation.
Recombinant APLP2 protein is engineered for in vitro studies to bypass challenges in isolating native forms from biological tissues. Produced via heterologous expression systems (e.g., mammalian cells, bacteria, or insect cells), the recombinant protein often includes tags (e.g., His-tag, Fc-fusion) to facilitate purification and detection. Researchers optimize conditions to ensure proper folding and post-translational modifications, critical for functional studies.
APLP2’s recombinant form enables exploration of its roles in neurodevelopment, neurodegeneration, and cancer. Studies using APLP2 knockout mice reveal subtle synaptic defects, suggesting compensatory mechanisms with APP/APLP1. Its involvement in metalloprotease activity and cell motility further links it to tumor progression. Additionally, recombinant APLP2 serves as a tool to identify binding partners or screen therapeutic agents targeting APP family proteins. Despite lacking direct amyloidogenicity, APLP2’s overlap with APP pathways makes it a valuable subject for understanding disease mechanisms and developing broad-spectrum therapies.
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