| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPRH |
| Uniprot No | Q9HD43 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1115aa |
| 氨基酸序列 | MAGAGGGLGVWGNLVLLGLCSWTGARAPAPNPGRNLTVETQTTSSISLSWEVPDGLDSQNSNYWVQCTGDGGTTETRNTTATNVTVDGLGPGSLYTCSVWVEKDGVNSSVGTVTTATAPNPVRNLRVEAQTNSSIALTWEVPDGPDPQNSTYGVEYTGDGGRAGTRSTAHTNITVDGLEPGCLYAFSMWVGKNGINSSRETRNATTAHNPVRNLRVEAQTTSSISLSWEVPDGTDPQNSTYCVQCTGDGGRTETRNTTDTRVTVDGLGPGSLYTCSVWVEKDGVNSSVEIVTSATAPNPVRNLTVEAQTNSSIALTWEVPDGPDPQNSTYGVEYTGDGGRAGTRSTAHTNITVDRLEPGCLYVFSVWVGKNGINSSRETRNATTAPNPVRNLHMETQTNSSIALCWEVPDGPYPQDYTYWVEYTGDGGGTETRNTTNTSVTAERLEPGTLYTFSVWAEKNGARGSRQNVSISTVPNAVTSLSKQDWTNSTIALRWTAPQGPGQSSYSYWVSWVREGMTDPRTQSTSGTDITLKELEAGSLYHLTVWAERNEVRGYNSTLTAATAPNEVTDLQNETQTKNSVMLWWKAPGDPHSQLYVYWVQWASKGHPRRGQDPQANWVNQTSRTNETWYKVEALEPGTLYNFTVWAERNDVASSTQSLCASTYPDTVTITSCVSTSAGYGVNLIWSCPQGGYEAFELEVGGQRGSQDRSSCGEAVSVLGLGPARSYPATITTIWDGMKVVSHSVVCHTESAGVIAGAFVGILLFLILVGLLIFFLKRRNKKKQQKPELRDLVFSSPGDIPAEDFADHVRKNERDSNCGFADEYQQLSLVGHSQSQMVASASENNAKNRYRNVLPYDWSRVPLKPIHEEPGSDYINASFMPGLWSPQEFIATQGPLPQTVGDFWRLVWEQQSHTLVMLTNCMEAGRVKCEHYWPLDSQPCTHGHLRVTLVGEEVMENWTVRELLLLQVEEQKTLSVRQFHYQAWPDHGVPSSPDTLLAFWRMLRQWLDQTMEGGPPIVHCSAGVGRTGTLIALDVLLRQLQSEGLLGPFSFVRKMRESRPLMVQTEAQYVFLHQCILRFLQQSAQAPAEKEVPYEDVENLIYENVAAIQAHKLEV |
| 预测分子量 | 122,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPRH重组蛋白的3篇参考文献及其摘要概括：
1. **标题**: "Crystal structure of human receptor-type tyrosine-protein phosphatase H (PTPRH)"
**作者**: Nam HJ et al.
**摘要**: 本研究解析了人源PTPRH重组蛋白胞外段的晶体结构，揭示了其纤维连接蛋白III型重复结构域的空间构象，为研究其与配体相互作用及肿瘤相关功能提供结构基础。
2. **标题**: "Expression and purification of the catalytic domain of PTPRH in Escherichia coli"
**作者**: Zhang Y et al.
**摘要**: 文章报道了利用大肠杆菌表达系统高效表达并纯化PTPRH的催化结构域重组蛋白，通过酶活实验验证其磷酸酶活性，为后续抑制剂筛选奠定技术基础。
3. **标题**: "PTPRH promotes tumor metastasis through regulating epithelial-mesenchymal transition in colorectal cancer"
**作者**: Wang L et al.
**摘要**: 该研究通过哺乳动物细胞表达重组PTPRH蛋白，发现其通过去磷酸化EGFR调控结直肠癌细胞上皮间质转化(EMT)，揭示了其在肿瘤转移中的分子机制。
注：以上文献信息为示例性内容，实际研究中建议通过PubMed或Web of Science检索具体文献。
**Background of PTPRH Recombinant Protein**
Protein tyrosine phosphatase receptor type H (PTPRH) is a member of the protein tyrosine phosphatase (PTP) family, which plays critical roles in regulating cellular signaling by dephosphorylating tyrosine residues on target proteins. As a transmembrane receptor-like PTP, PTPRH consists of an extracellular segment, a single transmembrane domain, and an intracellular catalytic domain. Its extracellular region contains fibronectin type III-like domains, suggesting potential interactions with extracellular ligands or matrix components, though its precise ligands remain under investigation.
PTPRH is implicated in modulating cell adhesion, growth, and differentiation, particularly in epithelial tissues. Studies highlight its role as a tumor suppressor in certain cancers, where its expression is often downregulated. For instance, PTPRH negatively regulates oncogenic signaling pathways, such as EGFR (epidermal growth factor receptor) signaling, by dephosphorylating key tyrosine residues, thereby inhibiting uncontrolled cell proliferation and migration. Dysregulation of PTPRH has been associated with colorectal, gastric, and pancreatic cancers, making it a potential biomarker or therapeutic target.
Recombinant PTPRH protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional protein for research. It enables the study of PTPRH’s enzymatic activity, structure-function relationships, and interactions with signaling molecules. Applications include in vitro binding assays, enzymatic kinetics analysis, and screening for inhibitors or activators. The recombinant form is often validated for phosphatase activity using substrates like para-nitrophenyl phosphate (pNPP) or phosphopeptides, ensuring its utility in mechanistic and drug discovery studies.
Overall, PTPRH recombinant protein serves as a vital tool for deciphering its biological roles and exploring therapeutic strategies targeting tyrosine phosphatase-mediated pathways in disease.
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