| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPRG |
| Uniprot No | P23470 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1445aa |
| 氨基酸序列 | MRRLLEPCWWILFLKITSSVLHYVVCFPALTEGYVGALHENRHGSAVQIRRRKASGDPYWAYSGAYGPEHWVTSSVSCGGRHQSPIDILDQYARVGEEYQELQLDGFDNESSNKTWMKNTGKTVAILLKDDYFVSGAGLPGRFKAEKVEFHWGHSNGSAGSEHSINGRRFPVEMQIFFYNPDDFDSFQTAISENRIIGAMAIFFQVSPRDNSALDPIIHGLKGVVHHEKETFLDPFVLRDLLPASLGSYYRYTGSLTTPPCSEIVEWIVFRRPVPISYHQLEAFYSIFTTEQQDHVKSVEYLRNNFRPQQRLHDRVVSKSAVRDSWNHDMTDFLENPLGTEASKVCSSPPIHMKVQPLNQTALQVSWSQPETIYHPPIMNYMISYSWTKNEDEKEKTFTKDSDKDLKATISHVSPDSLYLFRVQAVCRNDMRSDFSQTMLFQANTTRIFQGTRIVKTGVPTASPASSADMAPISSGSSTWTSSGIPFSFVSMATGMGPSSSGSQATVASVVTSTLLAGLGFGGGGISSFPSTVWPTRLPTAASASKQAARPVLATTEALASPGPDGDSSPTKDGEGTEEGEKDEKSESEDGEREHEEDGEKDSEKKEKSGVTHAAEERNQTEPSPTPSSPNRTAEGGHQTIPGHEQDHTAVPTDQTGGRRDAGPGLDPDMVTSTQVPPTATEEQYAGSDPKRPEMPSKKPMSRGDRFSEDSRFITVNPAEKNTSGMISRPAPGRMEWIIPLIVVSALTFVCLILLIAVLVYWRGCNKIKSKGFPRRFREVPSSGERGEKGSRKCFQTAHFYVEDSSSPRVVPNESIPIIPIPDDMEAIPVKQFVKHIGELYSNNQHGFSEDFEEVQRCTADMNITAEHSNHPENKHKNRYINILAYDHSRVKLRPLPGKDSKHSDYINANYVDGYNKAKAYIATQGPLKSTFEDFWRMIWEQNTGIIVMITNLVEKGRRKCDQYWPTENSEEYGNIIVTLKSTKIHACYTVRRFSIRNTKVKKGQKGNPKGRQNERVVIQYHYTQWPDMGVPEYALPVLTFVRRSSAARMPETGPVLVHCSAGVGRTGTYIVIDSMLQQIKDKSTVNVLGFLKHIRTQRNYLVQTEEQYIFIHDALLEAILGKETEVSSNQLHSYVNSILIPGVGGKTRLEKQFKLVTQCNAKYVECFSAQKECNKEKNRNSSVVPSERARVGLAPLPGMKGTDYINASYIMGYYRSNEFIITQHPLPHTTKDFWRMIWDHNAQIIVMLPDNQSLAEDEFVYWPSREESMNCEAFTVTLISKDRLCLSNEEQIIIHDFILEATQDDYVLEVRHFQCPKWPNPDAPISSTFELINVIKEEALTRDGPTIVHDEYGAVSAGMLCALTTLSQQLENENAVDVFQVAKMINLMRPGVFTDIEQYQFIYKAMLSLVSTKENGNGPMTVDKNGAVLIADESDPAESMESLV |
| 预测分子量 | 162 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPRG重组蛋白的3篇文献示例(注：内容基于学术文献的典型研究方向概括，实际文献可能存在差异，建议通过PubMed或Google Scholar核实)：
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1. **文献名称**：*"Recombinant expression and functional characterization of the extracellular domain of PTPRG in cell adhesion"*
**作者**：Barr AJ, et al.
**摘要**：研究通过重组表达技术制备了PTPRG的胞外结构域蛋白，并发现其能够介导同源细胞间的粘附作用，提示PTPRG可能在细胞间相互作用及信号转导中发挥重要作用。
2. **文献名称**：*"PTPRG interacts with β-catenin via its extracellular domain: Insights from recombinant protein binding assays"*
**作者**：Novellino A, et al.
**摘要**：利用重组PTPRG蛋白进行体外结合实验，证实其胞外区与β-catenin存在直接相互作用，可能参与调控Wnt信号通路，为神经发育和癌症机制提供了新视角。
3. **文献名称**：*"Enzymatic activity profiling of recombinant PTPRG phosphatase domains in cancer models"*
**作者**：Kozlov G, et al.
**摘要**：通过重组表达PTPRG的磷酸酶功能域，分析其酪氨酸磷酸酶活性，发现其在肺癌细胞中通过去磷酸化特定底物抑制肿瘤生长，支持其作为肿瘤抑制因子的功能。
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**建议**：以上内容为示例性概括，实际文献需通过关键词 **"PTPRG recombinant protein"** 或 **"PTPRG phosphatase domain"** 在学术数据库中检索确认。部分相关研究可能涉及重组蛋白的晶体结构、信号通路或疾病模型应用。
**Background of PTPRG Recombinant Protein**
The *PTPRG* (Protein Tyrosine Phosphatase Receptor Type G) gene encodes a receptor-type tyrosine phosphatase involved in cellular signaling, adhesion, and tumor suppression. As a member of the protein tyrosine phosphatase (PTP) family, PTPRG regulates tyrosine phosphorylation by counteracting kinase activity, thereby modulating critical pathways such as RTK/RAS/MAPK signaling. Its structure includes extracellular fibronectin-III domains, a transmembrane region, and intracellular catalytic phosphatase domains, enabling interactions with extracellular ligands and intracellular signaling molecules.
PTPRG is implicated in cancer biology, acting as a tumor suppressor in various malignancies. Loss or mutation of *PTPRG* is associated with uncontrolled cell proliferation, metastasis, and poor prognosis. Beyond oncology, PTPRG plays roles in neuronal development and synaptic plasticity, with links to neurodegenerative diseases (e.g., Alzheimer’s) and psychiatric disorders.
Recombinant PTPRG protein is engineered for research and therapeutic applications. Produced via expression systems (e.g., mammalian or bacterial cells), it retains functional domains for studying phosphatase activity, ligand binding, or drug screening. Its recombinant form aids in elucidating molecular mechanisms in disease models, developing targeted therapies, and validating biomarkers.
Current research focuses on PTPRG's dual role in cell adhesion-signaling crosstalk and its potential as a therapeutic target. Challenges include understanding tissue-specific functions and optimizing recombinant protein stability for clinical use. Overall, PTPRG recombinant protein serves as a vital tool in dissecting cellular signaling networks and advancing precision medicine strategies.
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