关键信息
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基因名
CD14
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简介
CD14 protein is a key protein in the innate immune response and is significantly expressed in monocytes/macrophages. It acts as a coreceptor that binds various microbial and fungal molecules, including lipopolysaccharide (LPS). CD14 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD14 protein, expressed by HEK293 , with C-6*His labeled tag.
- 应用
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生物活性
Measured by its ability to enhance LPS-induced IL-6 secretion by CTLL-2 cells. The ED50 this effect is 1.121 μg/mL, corresponding to a specific activity is 892.06 U/mg. Measured by its ability to enhance LPS-induced IL-6 secretion by CTLL -2 cells. The ED50 this effect is 1.121 μg/ml, corresponding to a specific activity is 892.06 U/mg.
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别名
rHuCD14, His; Monocyte Differentiation Antigen CD14; Myeloid Cell-Specific Leucine-Rich Glycoprotein; CD14
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种属
Mouse
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
P10810
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表达区间
S16-P345
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氨基酸序列
MERVLGLLLLLLVHASPAPPEPCELDEESCSCNFSDPKPDWSSAFNCLGAADVELYGGGRSLEYLLKRVDTEADLGQFTDIIKSLSLKRLTVRAARIPSRILFGALRVLGISGLQELTLENLEVTGTAPPPLLEATGPDLNILNLRNVSWATRDAWLAELQQWLKPGLKVLSIAQAHSLNFSCEQVRVFPALSTLDLSDNPELGERGLISALCPLKFPTLQVLALRNAGMETPSGVCSALAAARVQLQGLDLSHNSLRDAAGAPSCDWPSQLNSLNLSFTGLKQVPKGLPAKLSVLDLSYNRLDRNPSPDELPQVGNLSLKGNPFLDSESHSEKFNSGVVTAGAPSSQAVALSGTLALLLGDRLFV
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蛋白长度
Partial
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分子量
45-50 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CD14 is a glycoprotein primarily expressed on the surface of monocytes and macrophages, playing a critical role in the immune response by functioning as a co-receptor for the detection of bacterial lipopolysaccharides (LPS). As part of the innate immune system, CD14 aids in the recognition of pathogens, facilitating the activation of various immune pathways, including the production of pro-inflammatory cytokines. The study of CD14 recombinant protein has garnered significant attention due to its potential applications in immunology and therapeutic interventions. Researchers have explored its role in modulating immune responses, its interaction with other receptors, and its participation in various inflammatory diseases. By generating recombinant CD14, scientists can investigate its biochemical properties, assess its binding affinities, and evaluate its effects on immune cell activation. Furthermore, CD14's involvement in conditions such as sepsis, chronic inflammatory diseases, and autoimmune disorders highlights its clinical relevance, paving the way for potential therapeutic strategies. The advancement of recombinant technology has enabled more efficient production of CD14 protein, allowing for detailed studies and the development of CD14-based therapies or diagnostics, thus providing valuable insights into immune regulation and potential treatments for inflammatory diseases.
