| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UQCR |
| Uniprot No | O14957 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-56aa |
| 氨基酸序列 | MVTRFLGPRYRELVKNWVPTAYTWGAVGAVGLVWATDWRLILDWVPYINGKFKKDN |
| 预测分子量 | 33.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UQCR(泛醌-细胞色素c还原酶)重组蛋白的3-4篇参考文献示例(内容为模拟概括，非真实文献)：
1. **"Recombinant expression and functional characterization of human UQCRB in Escherichia coli"**
- **作者**: Smith J, et al. (2018)
- **摘要**: 本研究成功在大肠杆菌中表达了重组人源UQCRB蛋白，并通过亲和层析纯化获得高纯度蛋白。功能实验表明，重组UQCRB能够恢复线粒体复合体III缺陷细胞的呼吸链活性。
2. **"Structural insights into UQCRC1-UQCRC2 dimer assembly using recombinant proteins"**
- **作者**: Johnson L, et al. (2020)
- **摘要**: 通过重组表达UQCRC1和UQCRC2蛋白，结合体外复合体组装实验，揭示了二者二聚化对线粒体复合体III稳定性的关键作用，并提出了其互作界面的分子机制。
3. **"Role of recombinant UQCRFS1 in mitochondrial dysfunction models"**
- **作者**: Lee S, et al. (2021)
- **摘要**: 利用昆虫细胞系统表达重组UQCRFS1蛋白，发现其能够改善线粒体复合体III缺陷引起的活性氧(ROS)累积，为相关神经退行性疾病研究提供工具。
4. **"Crystallographic analysis of UQCRQ subunit in complex III using recombinant protein technology"**
- **作者**: Brown K, et al. (2016)
- **摘要**: 通过重组UQCRQ蛋白的结晶学分析，首次解析了其在复合体III中的空间构象，揭示了其与细胞色素b亚基的结合模式。
(注：以上文献为示例，实际引用需根据具体研究方向检索真实数据库如PubMed、Web of Science等。)
UQCR (Ubiquinol-Cytochrome c Reductase) recombinant proteins are engineered variants of subunits from mitochondrial Complex III (cytochrome bc1 complex), a critical component of the electron transport chain (ETC). Complex III facilitates electron transfer from ubiquinol to cytochrome c while pumping protons across the mitochondrial inner membrane, contributing to ATP synthesis. Key UQCR subunits include UQCRB (subunit 7), UQCRC1 (core protein 1), and UQCRC2 (core protein 2), which stabilize the complex's structure and mediate interactions with other ETC components.
Recombinant UQCR proteins are synthesized in vitro using expression systems like E. coli or mammalian cells, often tagged for purification. These proteins enable detailed studies of Complex III assembly, function, and dysfunction linked to diseases. For instance, UQCRB mutations are associated with mitochondrial disorders, while its overexpression in cancers highlights roles in tumor metabolism. Recombinant forms allow biochemical assays, structural analyses (e.g., X-ray crystallography), and drug screening, particularly for inhibitors targeting cancer-specific Complex III activity.
Additionally, UQCR recombinants aid in exploring oxidative stress mechanisms and neurodegenerative diseases like Parkinson’s, where ETC defects contribute to pathology. Their use in antibody production and diagnostic tools further underscores their biomedical relevance. Despite challenges in preserving native conformation post-purification, advancements in expression systems continue to enhance their utility in both basic research and therapeutic development.
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