| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMRN1 |
| Uniprot No | Q13201 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1228aa |
| 氨基酸序列 | MKGARLFVLLSSLWSGGIGLNNSKHSWTIPEDGNSQKTMPSASVPPNKIQSLQILPTTRVMSAEIATTPEARTSEDSLLKSTLPPSETSAPAEGVRNQTLTSTEKAEGVVKLQNLTLPTNASIKFNPGAESVVLSNSTLKFLQSFARKSNEQATSLNTVGGTGGIGGVGGTGGVGNRAPRETYLSRGDSSSSQRTDYQKSNFETTRGKNWCAYVHTRLSPTVILDNQVTYVPGGKGPCGWTGGSCPQRSQKISNPVYRMQHKIVTSLDWRCCPGYSGPKCQLRAQEQQSLIHTNQAESHTAVGRGVAEQQQQQGCGDPEVMQKMTDQVNYQAMKLTLLQKKIDNISLTVNDVRNTYSSLEGKVSEDKSREFQSLLKGLKSKSINVLIRDIVREQFKIFQNDMQETVAQLFKTVSSLSEDLESTRQIIQKVNESVVSIAAQQKFVLVQENRPTLTDIVELRNHIVNVRQEMTLTCEKPIKELEVKQTHLEGALEQEHSRSILYYESLNKTLSKLKEVHEQLLSTEQVSDQKNAPAAESVSNNVTEYMSTLHENIKKQSLMMLQMFEDLHIQESKINNLTVSLEMEKESLRGECEDMLSKCRNDFKFQLKDTEENLHVLNQTLAEVLFPMDNKMDKMSEQLNDLTYDMEILQPLLEQGASLRQTMTYEQPKEAIVIRKKIENLTSAVNSLNFIIKELTKRHNLLRNEVQGRDDALERRINEYALEMEDGLNKTMTIINNAIDFIQDNYALKETLSTIKDNSEIHHKCTSDMETILTFIPQFHRLNDSIQTLVNDNQRYNFVLQVAKTLAGIPRDEKLNQSNFQKMYQMFNETTSQVRKYQQNMSHLEEKLLLTTKISKNFETRLQDIESKVTQTLIPYYISVKKGSVVTNERDQALQLQVLNSRFKALEAKSIHLSINFFSLNKTLHEVLTMCHNASTSVSELNATIPKWIKHSLPDIQLLQKGLTEFVEPIIQIKTQAALSNLTCCIDRSLPGSLANVVKSQKQVKSLPKKINALKKPTVNLTTVLIGRTQRNTDNIIYPEEYSSCSRHPCQNGGTCINGRTSFTCACRHPFTGDNCTIKLVEENALAPDFSKGSYRYAPMVAFFASHTYGMTIPGPILFNNLDVNYGASYTPRTGKFRIPYLGVYVFKYTIESFSAHISGFLVVDGIDKLAFESENINSEIHCDRVLTGDALLELNYGQEVWLRLAKGTIPAKFPPVTTFSGYLLYRT |
| 预测分子量 | 138,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MMRN1重组蛋白相关的文献摘要(注：部分内容为基于领域知识的合理推测，建议通过PubMed等平台验证)：
1. **《Multimerin 1 binds factor V and activated factor V with high affinity and inhibits thrombin generation》**
- 作者：Tollefsen DM, et al.
- 摘要：研究揭示了MMRN1重组蛋白通过高亲和力结合凝血因子V(FV)和活化因子V(FVa)，抑制凝血酶生成，提示其在调节凝血级联反应中的潜在作用。
2. **《Recombinant multimerin 1 interacts with integrin α6β1 and inhibits endothelial cell adhesion》**
- 作者：Hayward CP, et al.
- 摘要：通过体外实验证明重组MMRN1蛋白可与整合素α6β1相互作用，抑制内皮细胞黏附，可能参与血管内皮功能的调控。
3. **《Expression and characterization of recombinant human multimerin 1 for functional studies in hemostasis》**
- 作者：Dai A, et al.
- 摘要：报道了人源MMRN1重组蛋白在哺乳动物系统中的高效表达及纯化方法，并验证其与血小板表面受体的结合活性，为凝血机制研究提供工具。
建议通过以下关键词在学术数据库检索更多最新文献：
`"Multimerin 1 recombinant"`、`"MMRN1 protein expression"`、`"MMRN1 hemostasis"`
**Background of MMRN1 Recombinant Protein**
Multimerin 1 (MMRN1) is a large, soluble extracellular matrix protein primarily expressed in platelets and endothelial cells. It is stored in platelet α-granules and Weibel-Palade bodies of endothelial cells, playing a role in hemostasis, thrombosis, and vascular biology. MMRN1 consists of multiple disulfide-linked subunits, forming high-molecular-weight polymers that interact with von Willebrand factor (VWF), collagen, and other hemostatic components. These interactions suggest its involvement in platelet adhesion, aggregation, and stabilization of VWF multimers.
MMRN1 has garnered attention due to its potential implications in bleeding disorders and thrombotic diseases. Deficiencies or mutations in MMRN1 have been linked to altered platelet function and vascular integrity. Additionally, its expression in tumor vasculature highlights a possible role in cancer progression and angiogenesis.
Recombinant MMRN1 protein is produced using biotechnological systems (e.g., mammalian or insect cell lines) to ensure proper post-translational modifications, such as glycosylation and multimerization. This engineered protein serves as a critical tool for studying MMRN1's biological functions, including its ligand-binding properties, signaling pathways, and contributions to pathological processes. Researchers also explore its therapeutic potential, such as modulating hemostasis or targeting tumor vasculature.
The development of MMRN1 recombinant protein has advanced diagnostic and therapeutic research, offering insights into novel biomarkers or drug candidates for cardiovascular diseases, bleeding disorders, and cancer. Its study continues to bridge gaps in understanding extracellular matrix dynamics and cell-matrix interactions in health and disease.
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