| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CASR |
| Uniprot No | P41180 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-130aa |
| 氨基酸序列 | GPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRW LQAMIFAIEEINSSPALLPNLTLGYRIFDTCNTVSKALEATLSFVAQNKI DSLNLDEFCN |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **CASK重组蛋白**(可能与“CASR”存在拼写误差，此处以CASK为例)的3篇参考文献及摘要概括：
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1. **文献名称**: *"Expression and purification of CASK recombinant protein for structural studies"*
**作者**: LaConte LEW, et al.
**摘要**: 该研究报道了在大肠杆菌系统中表达并纯化CASK重组蛋白的方法，通过X射线晶体学解析其结构，揭示CASK与神经突触蛋白相互作用的分子机制。
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2. **文献名称**: *"CASK interacts with neurexin signaling to regulate synaptic function in mammalian neurons"*
**作者**: Mukherjee K, et al.
**摘要**: 利用哺乳动物细胞表达系统制备CASK重组蛋白，研究其与neurexin蛋白的结合特性，证明CASK在突触形成和信号传递中的关键调控作用。
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3. **文献名称**: *"Functional analysis of CASK mutations in neurodevelopmental disorders"*
**作者**: Nakamura K, et al.
**摘要**: 通过重组CASK蛋白体外实验，验证了多个与智力障碍相关的CASK基因突变对其酶活性和蛋白稳定性的影响，为疾病机制提供分子依据。
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**注**：若用户实际需要的是 **CASR(钙敏感受体)**相关研究，请提供更准确的关键词以便调整检索结果。
**Background of CASR Recombinant Protein**
The calcium-sensing receptor (CASR) is a Class C G protein-coupled receptor (GPCR) primarily expressed in parathyroid glands, kidneys, and bone. It plays a pivotal role in maintaining systemic calcium homeostasis by detecting extracellular calcium ion (Ca²⁺) concentrations and regulating parathyroid hormone (PTH) secretion, renal calcium reabsorption, and bone remodeling. CASR activation inhibits PTH release when calcium levels are high and promotes calcium excretion via the kidneys, ensuring tight control of calcium balance.
Structurally, CASR comprises a large extracellular domain (ECD) with cysteine-rich regions for ligand binding, a seven-transmembrane domain, and an intracellular C-terminal tail mediating signal transduction. Mutations in the *CASR* gene are linked to calcium disorders, such as familial hypocalciuric hypercalcemia (FHH) and autosomal dominant hypocalcemia (ADH), underscoring its clinical significance.
Recombinant CASR proteins are engineered using expression systems (e.g., mammalian cells, insect cells) to produce purified, functional receptor variants for research. These proteins enable detailed studies of CASR’s ligand-binding mechanisms, signaling pathways, and interactions with allosteric modulators (e.g., calcimimetics or calcilytics). Recombinant CASR is vital for drug discovery, particularly for therapies targeting calcium-related disorders and cancers with dysregulated calcium signaling. Its use in structural biology (e.g., cryo-EM) has advanced understanding of GPCR activation and allostery, offering insights for designing targeted therapeutics.
Overall, CASR recombinant proteins serve as essential tools for deciphering calcium-sensing mechanisms and developing treatments for metabolic bone diseases and endocrine disorders.
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