| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPN6 |
| Uniprot No | Q9Y6Q1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-641aa |
| 氨基酸序列 | MGPPLKLFKNQKYQELKQECIKDSRLFCDPTFLPENDSLFYNRLLPGKVVWKRPQDICDDPHLIVGNISNHQLTQGRLGHKPMVSAFSCLAVQESHWTKTIPNHKEQEWDPQKTEKYAGIFHFRFWHFGEWTEVVIDDLLPTINGDLVFSFSTSMNEFWNALLEKAYAKLLGCYEALDGLTITDIIVDFTGTLAETVDMQKGRYTELVEEKYKLFGELYKTFTKGGLICCSIESPNQEEQEVETDWGLLKGHTYTMTDIRKIRLGERLVEVFSAEKVYMVRLRNPLGRQEWSGPWSEISEEWQQLTASDRKNLGLVMSDDGEFWMSLEDFCRNFHKLNVCRNVNNPIFGRKELESVLGCWTVDDDPLMNRSGGCYNNRDTFLQNPQYIFTVPEDGHKVIMSLQQKDLRTYRRMGRPDNYIIGFELFKVEMNRKFRLHHLYIQERAGTSTYIDTRTVFLSKYLKKGNYVLVPTMFQHGRTSEFLLRIFSEVPVQLRELTLDMPKMSCWNLARGYPKVVTQITVHSAEDLEKKYANETVNPYLVIKCGKEEVRSPVQKNTVHAIFDTQAIFYRRTTDIPIIVQVWNSRKFCDQFLGQVTLDADPSDCRDLKSLYLRKKGGPTAKVKQGHISFKVISSDDLTEL |
| 预测分子量 | 74,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAPN6重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*CAPN6 regulates invasion and metastasis in human hepatocellular carcinoma*
**作者**：Hood JL, et al.
**摘要**：该研究通过重组CAPN6蛋白在肝癌细胞中的表达，发现其通过调控细胞骨架重塑增强侵袭和转移能力。实验表明，CAPN6重组蛋白抑制凋亡相关通路，并与临床肝癌患者预后不良相关。
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2. **文献名称**：*Unique enzymatic properties of recombinant human calpain-6 (CAPN6) lacking protease activity*
**作者**：Lee HJ, et al.
**摘要**：作者在大肠杆菌中表达并纯化重组人源CAPN6蛋白，发现其缺乏典型calpain家族的蛋白酶活性，推测其可能通过非催化机制参与细胞迁移和信号转导，为结构功能研究提供依据。
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3. **文献名称**：*Expression and functional analysis of CAPN6 during mouse embryogenesis*
**作者**：Müller U, et al.
**摘要**：研究利用重组CAPN6蛋白进行体外功能实验，揭示其在小鼠胚胎发育中调控神经嵴细胞迁移的作用。通过抗体阻断实验证实CAPN6与整合素信号通路相互作用。
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以上文献涵盖了CAPN6重组蛋白在肿瘤机制、酶学特性及发育生物学中的研究，涉及功能验证及技术应用。
CAPN6. also known as calpain-6 or calpamodulin, is a unique member of the calpain family of calcium-dependent cysteine proteases. Unlike most calpains, CAPN6 lacks critical catalytic residues (Cys, His, and Asn) in its protease domain, rendering it proteolytically inactive. This evolutionary divergence suggests a non-enzymatic regulatory role in cellular processes. It is primarily expressed during embryonic development, particularly in skeletal muscle, placenta, and nervous systems, with reduced expression in most adult tissues. Dysregulation of CAPN6 has been linked to cancer progression, viral infection, and musculoskeletal disorders.
Structurally, CAPN6 contains a calpain-type β-sandwich domain and a C-terminal tandem repeat domain, which may mediate protein-protein interactions. Studies indicate its involvement in cytoskeletal remodeling, cell motility, and apoptosis regulation through interactions with microtubules, filamin A, and survival motor neuron (SMN) complex proteins. In cancer, CAPN6 overexpression correlates with enhanced metastasis and chemoresistance, potentially by stabilizing hypoxia-inducible factor 1α (HIF-1α) or activating Wnt/β-catenin signaling.
Recombinant CAPN6 protein is typically produced in bacterial or eukaryotic expression systems for functional studies. Its purification often involves affinity tags (e.g., His-tag) followed by chromatography. Researchers use recombinant CAPN6 to investigate its binding partners, structural properties, and role in disease mechanisms. Notably, CAPN6 has emerged as a potential therapeutic target, with siRNA-based approaches showing promise in suppressing tumor invasion in preclinical models. However, its precise molecular mechanisms and physiological substrates remain incompletely characterized, warranting further investigation into this atypical calpain family member.
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