Recombinant Human CR protein

中文名： 钙结合蛋白(CR)重组蛋白
别名： CR；Complement component receptor 1-like protein

货号: PA1000-8341

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	CR
Uniprot No	P17927
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	全长
氨基酸序列	full
预测分子量	kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于CR重组蛋白的3篇参考文献概览：

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1. **文献名称**：*Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1*

**作者**：Sauer, B., & Henderson, N.

**摘要**：该研究详细描述了噬菌体P1来源的Cre重组酶在哺乳动物细胞中介导loxP位点特异性重组的应用。通过实验验证了Cre在真核系统中高效切割与重组的特性，为基因编辑工具开发奠定基础。

2. **文献名称**：*CRISPR-Cas9 Structures and Mechanisms*

**作者**：Jinek, M., Chylinski, K., Fonfara, I., et al.

**摘要**：本文解析了Cas9重组蛋白的分子结构及其在CRISPR基因编辑中的作用机制，揭示了其通过RNA引导靶向DNA并产生双链断裂的功能，为设计高特异性基因编辑工具提供理论支持。

3. **文献名称**：*High-level expression of recombinant proteins in Escherichia coli using an optimized protein expression plasmid*

**作者**：Studier, F. W., & Moffatt, B. A.

**摘要**：研究提出一种优化的大肠杆菌重组蛋白表达系统，通过调控T7启动子与宿主菌株的协同作用显著提升蛋白产量，为规模化生产CR重组蛋白(如Cas9)提供技术参考。

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**注**：若“CR重组蛋白”特指某一细分领域(如CRISPR相关)，建议补充关键词以便提供更精准的文献。

背景信息

**Background of CR Recombinant Proteins**

CR recombinant proteins, primarily derived from the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, represent a groundbreaking advancement in genetic engineering and molecular biology. Originally discovered as a bacterial adaptive immune mechanism, CRISPR-associated (Cas) proteins, such as Cas9. were repurposed as programmable tools for precise genome editing. CR recombinant proteins typically refer to engineered variants of these Cas enzymes or fusion proteins combining Cas with functional domains (e.g., base editors, reverse transcriptases) to expand their capabilities beyond DNA cleavage.

The development of CR recombinant proteins was driven by the need to improve precision and versatility in genome manipulation. Traditional CRISPR-Cas9 induces double-strand breaks (DSBs), which can lead to unintended insertions, deletions, or chromosomal rearrangements. To address this, recombinant proteins like Cas9 nickases, dead Cas9 (dCas9) fused with effector domains (e.g., transcriptional activators), and base editors (e.g., BE4max) were engineered to enable targeted single-base edits, epigenetic modulation, or gene regulation without DSBs.

Applications span biomedical research, therapeutics, and agriculture. For instance, base-editing CR proteins correct point mutations in genetic disorders, while prime editors enable small DNA insertions or deletions. In agriculture, they enhance crop resilience by modifying traits like drought tolerance.

Challenges remain, including off-target effects, delivery efficiency, and ethical concerns. Ongoing research focuses on optimizing protein specificity, developing smaller Cas variants (e.g., Cas12f) for easier delivery, and exploring novel functionalities. CR recombinant proteins continue to revolutionize genetic research, offering unprecedented control over biological systems and paving the way for next-generation therapies and sustainable solutions.